Peptone Yeast Extract Broth with Glucose FA/GLC (PYG FA/GLC) is designed for the determination of glucose fermentation by anaerobic bacteria for identification. PYG FA/GLC is recommended for use in identifying gram-negative bacteria within the MIDI MIS procedures.
Peptone Yeast Extract Broth with Glucose FA/GLC (PYG FA/GLC)
Product Description & Summary
PYG FA/GLC is a non-selective broth medium that was formulated by the VPI group for use in chromatographic analysis of cellular fatty acids within the MIDI MIS system. For short chain fatty acid end products from the fermentation of glucose, appropriate control strains cultured in this medium will show characteristic metabolic products when analyzed using gas chromatography. Both analysis methods are useful in the identification of clinically significant anaerobic bacteria. This media is prepared, dispensed, and packaged under oxygen-free conditions to prevent the formation of oxidized products prior to use.
Interpretation of Results & Limitations
Uninoculated PY-based broths should show only trace amounts, if any, of volatile and nonvolatile fatty acids when tested with gas liquid chromatography. If used properly, this medium, when cultured with appropriate control strains, will show characteristic metabolic products when analyzed using MIDI MIS system. For interpretation of chromatographic results, consult the MIDI MIS manual.
PYG FF FA/GLC will not provide complete information for identification of bacterial isolates. Additional test procedures and media are required for complete identification. Consult reference materials for additional information.
The following organisms are routinely used for quality assurance performance testing, for growth and the fermentation of glucose, at Anaerobe Systems. Glucose utilization is determined on the basis of pH with the production of acid. A strong acid will result in a pH of 5.5 or below, weak acid results in a pH of 5.5 – 6.0, and no acid production results in a pH of 6.0 or above.
|Organism Tested||ATCC #
|Bacteroides fragilis*||25285||Growth||24 hrs||+|
|Prevotella melaninogenica||25845||Growth||24 – 48 hrs||NT|
|Bacteroides vulgatus||8482||Growth||24 hrs||NT|
|Fusobacterium nucleatum||25586||Growth||24 – 48 hrs||NT|
|Fusobacterium necrophorum||25286||Growth||24 – 48 hrs||–|
|Clostridium perfringens||13124||Growth||24 hrs||NT|
|Peptostreptococcus anaerobius||27337||Growth||24 hrs||NT|
|Peptoniphilus asaccharolyticus||29743||Growth||24 – 48 hrs||–|
|Propionibacterium acnes||6919||Growth||24 – 48 hrs||+|
|Staphylococcus aureus||25923||Growth||24 hrs||NT|
|Clostridium novyi||7659||Growth||48 hrs||NT|
*This organism is tested for characteristic fatty acid results using the MIDI MIS system. NT = Not Tested
Product Storage & Shelf Life
Storage: Upon receipt, store at room temperature in original package until used. Avoid overheating or freezing. Do not use media if there are signs of deterioration (shrinking, cracking, or discoloration due to oxidation of media) or contamination. The expiration date applies to the product in its original packaging and stored as directed. Do not use product past the expiration date shown on the label.
Shelf Life: 1 year from date of manufacture.