Campylobacter-Wolinella Differential Medium (CAMPY-WOL) is an enriched, selective, and differential media used for the isolation and presumptive identification of anaerobic Campylobacter (formerly Wolinella) species.
Campylobacter-Wolinella Differential Agar* – CAMPY-WOL
Product Description & Summary
CAMPY-WOL contains vancomycin at a concentration that inhibits most gram-positive organisms. This media is supplemented with ferrous sulfate as a source of iron. Sodium thiosulfate is included for a source of hydrogen sulfide production, which is visualized by the formation of black precipitation around the colonies for the differentiation of anaerobic Campylobacter spp. from other organisms. This media is prepared, dispensed, and packaged under oxygen-free conditions to prevent the formation of oxidized products prior to use.
Interpretation of Results & Limitations
If used properly, CAMPY-WOL supports good growth of Campylobacter (formerly Wolinella) species found in clinical infections.
CAMPY-WOL will not provide complete information for identification of bacterial isolates. Additional test procedures and media are required for complete identification. It is recommended that a non-selective media, such as Brucella Blood Agar (BRU, catalog #: AS-111) also be inoculated from the same clinical specimen to assure recovery of all species present. Additional test procedures and media are required for complete identification. Consult reference materials for additional information.
The following organisms are routinely used for quality assurance performance testing at Anaerobe Systems.
|Organism Tested||ATCC #||Results||Time|
|Campylobacter rectus||33238||Growth||24 hrs|
|Clostridium perfringens||13124||No Growth|
|Actinomyces viscosus||43146||No Growth|
|Campylobacter gracilis||33236||Growth||24 – 48 hrs|
|Bacteroides fragilis||25285||Growth||24 – 48 hrs|
|Peptostreptococcus anaerobius||27337||No Growth|
Product Storage & Shelf Life
Storage: Upon receipt, store at room temperature in original package until used. Avoid overheating or freezing. Do not use media if there are signs of deterioration (shrinking, cracking, or discoloration due to oxidation of media) or contamination. The expiration date applies to the product in its original packaging and stored as directed. Do not use product past the expiration date shown on the label.
Shelf Life: 90 days from date of manufacture.