Chamber surfaces should be cleaned after every manipulation of bacterial cultures. Before exiting the chamber, use a squirt bottle and a paper towel or cleaning cloth to wipe the chamber surfaces with benzalkonium chloride (AS-972 Chamber Cleaner). The chamber surfaces should be left damp, do not wipe it dry.
On a periodic basis, Clorox Healthcare Bleach Germicidal Wipes can be used, which will eradicate C. difficile spores with a 3 minute contact time. This should not be done more than once per day. Care must be taken when using the wipes. A single wipe should be removed and then the container tightly sealed. Wipe down the surfaces, immediately dispose of the wipe into the passbox, and make sure the passbox door is sealed shut to prevent excess bleach fumes from building inside chamber. After the Clorox specified contact time, wipe up all remaining wet spots and dispose of in the passbox.