Brain Heart Infusion Broth (BHI BROTH) is an enriched non-selective media intended for the cultivation of most anaerobic bacteria and other fastidious microorganisms.
Brain Heart Infusion Broth – BHI
Product Description & Summary
BHI BROTH is a general-purpose enriched non-selective broth media that is useful in the cultivation of fastidious and non-fastidious microorganisms from a variety of clinical and non-clinical specimens. It is also used for the preparation of inoculum for identification and susceptibility tests, and is especially useful as a blood culture medium. The basic nutritive properties of this media are brain heart infusion from solids and meat peptone. It is supplemented with vitamin K1 and hemin as growth factors for most anaerobes. This media is prepared, dispensed, and packaged under oxygen-free conditions to prevent the formation of oxidized products prior to use.
Interpretation of Results & Limitations
This media supports good growth of many fastidious and non-fastidious anaerobes isolated from clinical specimens.
BHI BROTH will not provide complete information for identification of bacterial isolates. Additional test procedures and media are required for complete identification. Consult reference materials for additional information.
The following organisms are routinely used for quality control testing at Anaerobe Systems.
|Organism Tested||ATCC #
|Bacteroides fragilis||25285||Growth||24 hrs|
|Prevotella melaninogenica||25845||Growth||24 – 48 hrs|
|Bacteroides vulgatus||8482||Growth||24 hrs|
|Fusobacterium nucleatum||25586||Growth||24 hrs|
|Fusobacterium necrophorum||25286||Growth||24 – 48 hrs|
|Clostridium perfringens||13124||Growth||24 hrs|
|Clostridium novyi||7659||Growth||24 – 48 hrs|
|Peptostreptococcus anaerobius||27337||Growth||24 hrs|
|Clostridium difficile||9689||Growth||24 hrs|
|Staphylococcus aureus||25923||Growth||24 hrs|
Product Storage & Shelf Life
Storage: Upon receipt, store at room temperature in original package until used. Avoid overheating or freezing. Do not use media if there are signs of deterioration (discoloration due to oxidation of media) or contamination. The expiration date applies to the product in its original packaging and stored as directed. Do not use product past the expiration date shown on the label.
Shelf Life: 1 year from date of manufacture.