Brain Heart Infusion Broth – BHI

Brain Heart Infusion Broth – BHI

Brain Heart Infusion Broth (BHI BROTH) is an enriched non-selective media intended for the cultivation of most anaerobic bacteria and other fastidious microorganisms.

BHI Broth

BHI Broth Packaging

Large Bottle Options

Catalog #
AS-872 Brain Heart Infusion Broth – BHI BROTH 10 tubes / pkg $17.42
AS-8722 BHI Broth, 250mL 1 Bottle
AS-8725 BHI Broth, 500mL 1 Bottle

Non-stock item:
AS-8722 – 10 bottle minimum order, allow 2-4 weeks for delivery.
AS-8725 – 5 bottle minimum order, allow 2-4 weeks for delivery.


Product Description & Summary

BHI BROTH is a general-purpose enriched non-selective broth media that is useful in the cultivation of fastidious and non-fastidious microorganisms from a variety of clinical and non-clinical specimens. It is also used for the preparation of inoculum for identification and susceptibility tests, and is especially useful as a blood culture medium. The basic nutritive properties of this media are brain heart infusion from solids and meat peptone. It is supplemented with vitamin K1 and hemin as growth factors for most anaerobes. This media is prepared, dispensed, and packaged under oxygen-free conditions to prevent the formation of oxidized products prior to use.

Interpretation of Results & Limitations

This media supports good growth of many fastidious and non-fastidious anaerobes isolated from clinical specimens.

BHI BROTH will not provide complete information for identification of bacterial isolates. Additional test procedures and media are required for complete identification. Consult reference materials for additional information.

Quality Control

The following organisms are routinely used for quality control testing at Anaerobe Systems.

Organism Tested ATCC #


Results Time
Bacteroides fragilis 25285 Growth 24 hrs
Prevotella melaninogenica 25845 Growth 24 – 48 hrs
Bacteroides vulgatus 8482 Growth 24 hrs
Fusobacterium nucleatum 25586 Growth 24 hrs
Fusobacterium necrophorum 25286 Growth 24 – 48 hrs
Clostridium perfringens 13124 Growth 24 hrs
Clostridium novyi 7659 Growth 24 – 48 hrs
Peptostreptococcus anaerobius 27337 Growth 24 hrs
Clostridium difficile 9689 Growth 24 hrs
Staphylococcus aureus 25923 Growth 24 hrs


Product Storage & Shelf Life

Storage: Upon receipt, store at room temperature in original package until used. Avoid overheating or freezing. Do not use media if there are signs of deterioration (discoloration due to oxidation of media) or contamination. The expiration date applies to the product in its original packaging and stored as directed. Do not use product past the expiration date shown on the label.

Shelf Life: 1 year from date of manufacture.