Brucella Broth (BRU BROTH) is an enriched non-selective media intended for the cultivation of most anaerobic bacteria and other fastidious microorganisms. This media will support the growth of aerobic and microaerophilic bacteria if incubated appropriately.
Brucella Broth – BRU-BROTH
Product Description & Summary
BRU BROTH is an enriched non-selective broth media. The basal media consists of casein and peptones to facilitate the growth of a wide variety of microorganisms. Yeast extract is provided as a source of trace vitamins and micronutrients. A small volume of dextrose is added as a carbohydrate source, to prevent acid build up. This media is supplemented with vitamin K1 and hemin to facilitate the recovery and growth of Prevotella melaninogenica. This media is prepared, stored, and dispensed under oxygen-free conditions to prevent the formation of oxidized products prior to use.
Interpretation of Results & Limitations
This media should support good growth of obligate anaerobes, aerobes, and microaerophilic microorganisms isolated from clinical specimens.
BRU BROTH will not provide complete information for identification of bacterial isolates. Additional test procedures and media are required for complete identification. In some cases, this media may not grow every anaerobic strain. Consult reference materials for additional information.
The following organisms are routinely used for quality control testing at Anaerobe Systems.
|Organism Tested||ATCC #||Results||Time|
|Bacteroides fragilis||25285||Growth||24 hrs|
|Bacteroides vulgatus*||8482||Growth||24 hrs|
|Bacteroides thetaiotaomicron||29741||Growth||24 hrs|
|Eggerthella lenta||43055||Growth||24 – 48 hrs|
|Prevotella melaninogenica||25845||Growth||24 – 48 hrs|
|Fusobacterium necrophorum||25286||Growth||24 hrs|
|Fusobacterium nucleatum||25586||Growth||24 hrs|
|Clostridium perfringens*||13124||Growth||24 hrs|
|Peptostreptococcus anaerobius*||27337||Growth||24 hrs|
|Staphylococcus aureus||25923||Growth||24 hrs|
|Clostridium novyi||7659||Growth||24 hrs|
|Propionibacterium acnes||6919||Growth||24 – 48 hrs|
* Organisms specified by CLSI for Quality Control testing of Anaerobic Broths.
Product Storage & Shelf Life
Storage:Upon receipt, store at room temperature in original package until used. Avoid overheating or freezing. Do not use media if there are signs of deterioration (discoloration due to oxidation of media) or contamination. The expiration date applies to the product in its original packaging and stored as directed. Do not use product past the expiration date shown on the label.
Shelf Life: 1 year from date of manufacture.