Campylobacter-Thioglycollate Medium (CAMPY-THIO) is used as a selective growth enrichment broth for Campylobacter species from contaminated sources.
Campylobacter-Thioglycollate Broth – CAMPY-THIO
Product Description & Summary
CAMPY-THIO media is used for the recovery and isolation of Campylobacter species. CAMPY-THIO is a basal media supplemented with antibiotics from the Blaser formulation. Minor changes have been made to the original formulation. Cephalothin has been removed, and the concentration of Polymyxin B has been increased to prevent overgrowth of other bacteria. A low concentration of agar has been added to slow the diffusion of oxygen within the media. This media is prepared, dispensed, and packaged under oxygen-free conditions to prevent the formation of oxidized products prior to use.
Interpretation of Results & Limitations
If used properly, this media aides in the isolation of Campylobacter species from clinical specimens contaminated by normal flora. Campylobacter jejuni subsp. jejuni, and Campylobacter fetus subsp. fetus should be recovered upon subculturing onto appropriate media.
CAMPY-THIO media will not provide complete information for identification of bacterial isolates. Additional test procedures and media are required for complete identification. Some organisms normally recovered from this media may be inhibited. Consult reference materials for additional information.
The following organisms are routinely used for quality assurance testing at Anaerobe Systems.
|Organism Tested||ATCC #
|Campylobacter jejuni subsp. jejuni||33291||Growth||24 – 48 hrs|
|Campylobacter fetus subsp. fetus||33246||Growth||24 – 48 hrs|
|Staphylococcus aureus||25923||No Growth|
|Enterococcus faecalis||29212||No Growth|
|Escherichia coli||25922||No Growth|
|Proteus mirabilis||12453||Inhibited to No Growth|
Product Storage & Shelf Life
Storage: Upon receipt, store at room temperature in original packaging until use. Avoid overheating or freezing. Do not use media if there are signs of deterioration (discoloration or evaporation) or contamination. The expiration date applies to the product in its original packaging and stored as directed. Do not use product past the expiration date shown on the container.
Shelf Life: 1 year from date of manufacture.