Chopped Meat Broth – CM

CHOPPED MEAT MEDIUM (CM, CMG, and CMC)

Chopped Meat Mediums (CM, CMG, and CMC) will support the growth of most non-sporeforming and sporeforming anaerobes associated with human and animal infections. This media may also be used as a maintaining or holding media for stock cultures.

Chopped Meat Broth

Chopped Meat Broth Packaging

Catalog #
Description
Qty
Price
AS-811 Chopped Meat Medium 10 tubes / pkg $14.99
AS-813 Chopped Meat Medium w/ Glucose (CMG)   10 tubes / pkg $14.99
AS-823 Chopped Meat Medium w/ Carbohydrates (CMC)         10 tubes / pkg $15.49
CM, CMG, CMC InsertCM SDS

Product Description & Summary

CM, CMG, and CMC are enriched non-selective medias that are useful as a holding media for stock and mixed cultures, sporulation, proteolysis, and toxin production by certain Clostridia spp. such as Clostridium novyi, Type A. These medias are used for preservation of clostridial cultures by freezing. CM, CMG, and CMC have the capacity to initiate growth from a minute inoculum and maintain viability of organisms over extended periods. These medias allow slower growing organisms, within a mixed sample, to proliferate in the presence of rapid reproducing organisms and are used to demonstrate clostridial toxin production, sporulation, and short chain organic acid production by gas chromatography. These medias are prepared, dispensed, and packaged under oxygen-free conditions to prevent the formation of oxidized products prior to use.

Interpretation of Results & Limitations

If used properly, Chopped Meat Mediums (CM, CMG, and CMC) supports good growth of Clostridium tetani, Clostridium sporogenes, Prevotella melaninogenica, and Fusobacterium necrophorum from a small inoculum (i.e. 0.01 ml of a 24 – 48 hour Lombard-Dowell (LD) Broth culture diluted to 3:1000). Both C. tetani and C. sporogenes should produce characteristic spores and C. sporogenes should exhibit typical proteolysis of the meat. In addition, C. septicum and C. tetani should exhibit typical toxin production as demonstrated by mouse toxicity and mouse toxin neutralization.

CM, CMG, and CMC will not provide complete information for the identification of bacterial isolates. Additional test procedures and media are required for complete identification. Consult reference materials for additional information.

Quality Control

The following organisms are routinely used for quality assurance testing at Anaerobe Systems.

Organism Tested ATCC #

 

Results Time
Bacteroides fragilis 25285 Growth 24 hrs
Prevotella melaninogenica 25845 Growth 24 – 48 hrs
Bacteroides vulgatus 8482 Growth 24 hrs
Fusobacterium nucleatum 25586 Growth 24 – 48 hrs
Fusobacterium necrophorum 25286 Growth 24 – 48 hrs
Clostridium perfringens 13124 Growth 24 hrs
Clostridium novyi 7659 Growth 24 – 48 hrs
Peptostreptococcus anaerobius 27337 Growth 24 hrs
Propionibacterium acnes 6919 Growth 24 – 48 hrs
Staphylococcus aureus 25923 Growth 24 hrs

Product Storage & Shelf Life

Storage:Upon receipt, store at room temperature in original packaging until use. Avoid overheating or freezing. Do not use media if there are signs of deterioration (discoloration or evaporation) or contamination. The expiration date applies to the product in its original packaging and stored as directed. Do not use product past the expiration date shown on the container.

Shelf Life: 1 year from date of manufacture.