Chopped Meat Mediums (CM, CMG, and CMC) will support the growth of most non-sporeforming and sporeforming anaerobes associated with human and animal infections. This media may also be used as a maintaining or holding media for stock cultures.
CHOPPED MEAT MEDIUM (CM, CMG, and CMC)
Product Description & Summary
CM, CMG, and CMC are enriched non-selective medias that are useful as a holding media for stock and mixed cultures, sporulation, proteolysis, and toxin production by certain Clostridia spp. such as Clostridium novyi, Type A. These medias are used for preservation of clostridial cultures by freezing. CM, CMG, and CMC have the capacity to initiate growth from a minute inoculum and maintain viability of organisms over extended periods. These medias allow slower growing organisms, within a mixed sample, to proliferate in the presence of rapid reproducing organisms and are used to demonstrate clostridial toxin production, sporulation, and short chain organic acid production by gas chromatography. These medias are prepared, dispensed, and packaged under oxygen-free conditions to prevent the formation of oxidized products prior to use.
Interpretation of Results & Limitations
If used properly, Chopped Meat Mediums (CM, CMG, and CMC) supports good growth of Clostridium tetani, Clostridium sporogenes, Prevotella melaninogenica, and Fusobacterium necrophorum from a small inoculum (i.e. 0.01 ml of a 24 – 48 hour Lombard-Dowell (LD) Broth culture diluted to 3:1000). Both C. tetani and C. sporogenes should produce characteristic spores and C. sporogenes should exhibit typical proteolysis of the meat. In addition, C. septicum and C. tetani should exhibit typical toxin production as demonstrated by mouse toxicity and mouse toxin neutralization.
CM, CMG, and CMC will not provide complete information for the identification of bacterial isolates. Additional test procedures and media are required for complete identification. Consult reference materials for additional information.
The following organisms are routinely used for quality assurance testing at Anaerobe Systems.
|Organism Tested||ATCC #
|Bacteroides fragilis||25285||Growth||24 hrs|
|Prevotella melaninogenica||25845||Growth||24 – 48 hrs|
|Bacteroides vulgatus||8482||Growth||24 hrs|
|Fusobacterium nucleatum||25586||Growth||24 – 48 hrs|
|Fusobacterium necrophorum||25286||Growth||24 – 48 hrs|
|Clostridium perfringens||13124||Growth||24 hrs|
|Clostridium novyi||7659||Growth||24 – 48 hrs|
|Peptostreptococcus anaerobius||27337||Growth||24 hrs|
|Propionibacterium acnes||6919||Growth||24 – 48 hrs|
|Staphylococcus aureus||25923||Growth||24 hrs|
Product Storage & Shelf Life
Storage:Upon receipt, store at room temperature in original packaging until use. Avoid overheating or freezing. Do not use media if there are signs of deterioration (discoloration or evaporation) or contamination. The expiration date applies to the product in its original packaging and stored as directed. Do not use product past the expiration date shown on the container.
Shelf Life: 1 year from date of manufacture.