Cycloserine Cefoxitin Mannitol Broth with Taurocholate and Lysozyme (CCMB-TAL) is an enriched, selective, and differential broth media used for the isolation and presumptive identification of Clostridium difficile, a recognized cause of pseudomembraneous (antimicrobial agent-associated) colitis.
Cycloserine Cefoxitin Mannitol Broth with Taurocholate Lysozyme Cysteine – CCMB-TAL
Product Description & Summary
CCMB-TAL is an enriched, selective, and differential broth media used for the isolation of Clostridium difficile. The nutritive base consists of animal peptones and mannitol. This media is supplemented with cefoxitin and cycloserine at concentrations that inhibit the growth of most normal fecal flora. Cycloserine will inhibit gram-negative bacteria, while cefoxitin will inhibit both gram-positive and gram-negative organisms. Clostridium difficile is not inhibited in the CCMB-TAL media, and when grown, will exhibit a characteristic smell. Neutral red is added as a pH indicator. The presence of C. difficile will turn the indicator from red/orange to yellow due to the organism utilizing the amino acids and causing an increase in the pH. Sodium Taurocholate and lysozyme are added to help in the germination of spores. Cysteine is added as a reducing agent. This media is prepared, dispensed, and packaged under oxygen-free conditions to prevent the formation of oxidized products prior to use.
Interpretation of Results & Limitations
If used properly, CCMB-TAL supports good growth of species of C. difficile. After 24-48 hours, the tube should be yellow in color from the increase in pH due to the utilization of amino acids by C. difficile. When recovered on plated media, these colonies fluoresce golden yellow/chartreuse under long-wavelength UV light. Most other bacteria are inhibited in this media. After 48 hours, other organisms such as Lactobacilli, Clostridia, and yeast, may grow and can increase the pH changing the indicator to yellow.
CCMB-TAL will not provide complete information for identification of bacterial isolates. Rare strains of C. difficile may be inhibited. Tubes should be examined after 24 and 48 hours of incubation to insure optimal selectivity. After 48 hours of incubation, significant numbers of isolates other than C. difficile may grow. A test for aerotolerance should be used to confirm that each colony type is an obligate anaerobe. Consult reference materials for additional information.
The following organisms are routinely used for quality control performance testing at Anaerobe Systems.
|Organism Tested||ATCC #||Results||Time||Special Reaction|
|Bacteroides fragilis||25285||No Growth|
|Enterococcus faecalis||29212||No Growth|
|Clostridium sporogenes||3584||No Growth|
|Clostridium beijerinckii||8260||No Growth|
|Proteus mirabilis||12453||No Growth|
|Clostridium perfringens||13124||No Growth|
|Clostridium innocuum||14501||No Growth|
|Clostridium sordellii||9714||No Growth|
|Clostridium difficile||9689||Growth||24 hrs||Yellow coloration|
|Clostridium difficile||700057||Growth||24 hrs||Yellow coloration|
Product Storage & Shelf Life
Storage: Upon receipt, store at 2 – 8oC in original container until used. Avoid overheating or freezing. Do not use media if there are signs of deterioration (shrinking, cracking, or discoloration due to oxidation of media) or contamination. The expiration date applies to the product in its original packaging and stored as directed. Do not use product past the expiration date shown on the container.
Shelf Life: 90 days from date of manufacture at 2 – 8oC.