Cycloserine Cefoxitin Mannitol Broth with Taurocholate Lysozyme Cysteine – CCMB-TAL

Cycloserine Cefoxitin Mannitol Broth with Taurocholate Lysozyme Cysteine – CCMB-TAL

Cycloserine Cefoxitin Mannitol Broth with Taurocholate and Lysozyme (CCMB-TAL) is an enriched, selective, and differential broth media used for the isolation and presumptive identification of Clostridium difficile, a recognized cause of pseudomembraneous (antimicrobial agent-associated) colitis.

CCMB-TAL Broth

C. difficile (ATCC 700057) Growing In CCMB-TAL Broth

AS-8216 PACKAGE

Catalog #
Description
Qty
Price
AS-8216 Cycloserine Cefoxitin Mannitol Broth with Taurocholate and Lysozyme (CCMB-TAL) 10 tubes / pkg $29.36
CCMB-TAL InsertCCMB-TAL SDS

Product Description & Summary

CCMB-TAL is an enriched, selective, and differential broth media used for the isolation of Clostridium difficile. The nutritive base consists of animal peptones and mannitol. This media is supplemented with cefoxitin and cycloserine at concentrations that inhibit the growth of most normal fecal flora. Cycloserine will inhibit gram-negative bacteria, while cefoxitin will inhibit both gram-positive and gram-negative organisms. Clostridium difficile is not inhibited in the CCMB-TAL media, and when grown, will exhibit a characteristic smell. Neutral red is added as a pH indicator. The presence of C. difficile will turn the indicator from red/orange to yellow due to the organism utilizing the amino acids and causing an increase in the pH. Sodium Taurocholate and lysozyme are added to help in the germination of spores. Cysteine is added as a reducing agent. This media is prepared, dispensed, and packaged under oxygen-free conditions to prevent the formation of oxidized products prior to use.

Interpretation of Results & Limitations

If used properly, CCMB-TAL supports good growth of species of C. difficile. After 24-48 hours, the tube should be yellow in color from the increase in pH due to the utilization of amino acids by C. difficile. When recovered on plated media, these colonies fluoresce golden yellow/chartreuse under long-wavelength UV light. Most other bacteria are inhibited in this media. After 48 hours, other organisms such as Lactobacilli, Clostridia, and yeast, may grow and can increase the pH changing the indicator to yellow.

CCMB-TAL will not provide complete information for identification of bacterial isolates. Rare strains of C. difficile may be inhibited. Tubes should be examined after 24 and 48 hours of incubation to insure optimal selectivity. After 48 hours of incubation, significant numbers of isolates other than C. difficile may grow. A test for aerotolerance should be used to confirm that each colony type is an obligate anaerobe. Consult reference materials for additional information.

Quality Control

The following organisms are routinely used for quality control performance testing at Anaerobe Systems.

Organism Tested ATCC # Results Time Special Reaction
Bacteroides fragilis 25285 No Growth
Enterococcus faecalis 29212 No Growth
Clostridium sporogenes 3584 No Growth
Clostridium beijerinckii 8260 No Growth
Proteus mirabilis 12453 No Growth
Clostridium perfringens 13124 No Growth
Clostridium innocuum 14501 No Growth
Clostridium sordellii 9714 No Growth
Clostridium difficile 9689 Growth 24 hrs Yellow coloration
Clostridium difficile 700057 Growth 24 hrs Yellow coloration

Product Storage & Shelf Life

Storage: Upon receipt, store at 2 – 8oC in original container until used. Avoid overheating or freezing. Do not use media if there are signs of deterioration (shrinking, cracking, or discoloration due to oxidation of media) or contamination. The expiration date applies to the product in its original packaging and stored as directed. Do not use product past the expiration date shown on the container.

Shelf Life: 90 days from date of manufacture at 2 – 8oC.