Lactobacillus MRS broth (LMRS BROTH) is an enriched selective medium for the isolation and cultivation of Lactobacillus found in clinical, dairy, and food specimens.
LACTOBACILLUS MRS BROTH (LMRS BROTH)
Product Description & Summary
Lactobacillus MRS (deMan, Rogosa, and Sharpe) broth is an enriched selective medium for the cultivation of Lactobacillus from clinical, dairy, and food specimens. LMRS broth consists of proteose peptone no. 3, beef extract, yeast extract, and dextrose as the nutritive base. The medium is supplemented with polysorbate 80 (Tween 80) and magnesium as a source of fatty acids and additional growth requirements. Sodium acetate and ammonium citrate may inhibit normal flora, such as gram-negative bacteria, oral flora, and fungi. Improved recovery of Lactobacilli occurs in the presence of these selective agents. The pH is adjusted to 6.3 to 6.7 to favor the growth of Lactobacillus spp. This medium is prepared, dispensed, and packaged under oxygen-free conditions to prevent the formation of oxidized products prior to use.
Interpretation of Results & Limitations
If used properly, LMRS broth will support the good growth of Lactobacillus from clinical and non-clinical specimens.
LMRS broth will not provide complete information for identification of bacterial isolates. Additional test procedures and media are required for complete identification. It is recommended that a non-selective media, such as Brucella Blood Agar (BRU, catalog #: AS-111) also be inoculated from the same clinical specimen to assure recovery of all species present. Consult reference materials for additional information.
The following organisms are routinely used for quality control testing at Anaerobe Systems.
|Organism Tested||ATCC #||Results||Time|
|Lactobacillus vaginalis||49540||Growth||24 – 48 hrs|
|Lactobacillus acidophilus||4356||Growth||24 – 48 hrs|
|Lactobacillus crispatus||33197||Growth||24 – 48 hrs|
|Lactobacillus jensenii||25258||Growth||24 – 48 hrs|
|Lactobacillus fermentum||9338||Growth||24 – 48 hrs|
Product Storage & Shelf Life
Storage: Upon receipt, store at room temperature in original package until used. Avoid overheating or freezing. Do not use media if there are signs of deterioration (shrinking, cracking, or discoloration due to oxidation of media) or contamination. The expiration date applies to the product in its original packaging and stored as directed. Do not use product past the expiration date shown on the label.
Shelf Life: 90 days from date of manufacture.