Oral Treponeme Enrichment Broth – OTEB

Oral Treponeme Enrichment Broth – OTEB

Oral Treponeme Enrichment Broth (OTEB) is an enriched nonselective medium used for the isolation, cultivation, and maintenance of Treponema spp. from clinical specimens.

OTEB Broth

OTEB Broth Packaging

Large Format Options

Catalog #
AS-603 Oral Treponeme Enrichment Broth – OTEB 10 tubes / pkg $27.26
AS-6032 OTEB Broth, 250mL 1 Bottle
AS-6035 OTEB Broth, 500mL 1 Bottle

Non-stock items:
AS-6032 – 10 bottle minimum order, allow 2-4 weeks for delivery.
AS-6035 – 5 bottle minimum order, allow 2-4 weeks for delivery.


Product Description & Summary

OTEB is a complex peptone yeast extract medium containing volatile fatty acids and calf serum. Volatile fatty acids and serum are necessary growth factors for the recovery of Treponemes from clinical specimens. A variety of sugars have been added as a source of carbohydrates. This medium is prepared, dispensed, and packaged under oxygen-free conditions to prevent the formation of oxidized products prior to use.

Interpretation of Results & Limitations

If used properly, OTEB will support good growth of most Treponema species found in clinical specimens.

OTEB will not provide complete information for identification of bacterial isolates. Additional test procedures and media are required for complete identification. Consult reference materials for additional information.

Quality Control

The following organisms are routinely used for quality control testing at Anaerobe Systems.

Organism Tested ATCC # Results Time
Treponema denticola 33521 Growth 72 – 96 hrs
Treponema phagedenis 27087 Growth 72 – 96 hrs
Bacteroides fragilis 25285 Growth 24 hrs
Bacteroides vulgatus 8482 Growth 24 hrs
Prevotella melaninogenica 25845 Growth 24 – 48 hrs
Fusobacterium necrophorum 25286 Growth 24 hrs
Fusobacterium nucleatum 25586 Growth 24 hrs
Clostridium perfringens 13124 Growth 24 hrs
Peptostreptococcus anaerobius 27337 Growth 24 hrs
Staphylococcus aureus 25923 Growth 24 hrs

Product Storage & Shelf Life

Storage: Upon receipt, store at room temperature in original package until used. Avoid overheating or freezing. Do not use media if there are signs of deterioration (discoloration due to oxidation of media) or contamination. The expiration date applies to the product in its original packaging and stored as directed. Do not use product past the expiration date shown on the label.

Shelf Life: 6 months from date of manufacture.