Oral Treponeme Enrichment Broth (OTEB) is an enriched nonselective medium used for the isolation, cultivation, and maintenance of Treponema spp. from clinical specimens.
Oral Treponeme Enrichment Broth – OTEB
Product Description & Summary
OTEB is a complex peptone yeast extract medium containing volatile fatty acids and calf serum. Volatile fatty acids and serum are necessary growth factors for the recovery of Treponemes from clinical specimens. A variety of sugars have been added as a source of carbohydrates. This medium is prepared, dispensed, and packaged under oxygen-free conditions to prevent the formation of oxidized products prior to use.
Interpretation of Results & Limitations
If used properly, OTEB will support good growth of most Treponema species found in clinical specimens.
OTEB will not provide complete information for identification of bacterial isolates. Additional test procedures and media are required for complete identification. Consult reference materials for additional information.
The following organisms are routinely used for quality control testing at Anaerobe Systems.
|Organism Tested||ATCC #||Results||Time|
|Treponema denticola||33521||Growth||72 – 96 hrs|
|Treponema phagedenis||27087||Growth||72 – 96 hrs|
|Bacteroides fragilis||25285||Growth||24 hrs|
|Bacteroides vulgatus||8482||Growth||24 hrs|
|Prevotella melaninogenica||25845||Growth||24 – 48 hrs|
|Fusobacterium necrophorum||25286||Growth||24 hrs|
|Fusobacterium nucleatum||25586||Growth||24 hrs|
|Clostridium perfringens||13124||Growth||24 hrs|
|Peptostreptococcus anaerobius||27337||Growth||24 hrs|
|Staphylococcus aureus||25923||Growth||24 hrs|
Product Storage & Shelf Life
Storage: Upon receipt, store at room temperature in original package until used. Avoid overheating or freezing. Do not use media if there are signs of deterioration (discoloration due to oxidation of media) or contamination. The expiration date applies to the product in its original packaging and stored as directed. Do not use product past the expiration date shown on the label.
Shelf Life: 6 months from date of manufacture.