Peptone Yeast Extract Broth – PY

Peptone Yeast Extract Broth – PY

Peptone Yeast Extract Broth Based Media are enriched nonselective media that are supplemented with hemin and vitamin K1 to facilitate the recovery of the more fastidious organisms such as Prevotella spp, Porphyromonas spp, and the Bacteroides fragilis group. Resazurin is added as a redox indicator. PRAS (Pre-reduced anaerobically sterilized) biochemicals are considered to be the “gold standard” for biochemical testing and characterization of anaerobes. With the addition of a specific biochemical, the Peptone Yeast Extract Broth Based Media are used to determine whether anaerobic bacteria will utilize the biochemical or if growth is stimulated or inhibited. PY and PYG are nonselective broth media originally formulated by the VPI group for use in chromatographic analysis of metabolic products from the fermentation of glucose. This chromatographic analysis is useful in the identification of clinically important anaerobic bacteria. This media is prepared, dispensed, and packaged under oxygen-free conditions to prevent the formation of oxidized products prior to use.

Peptone Yeast Broth

Peptone Yeast Broth Packaging

Catalog #
Description
Qty
Price
AS-821 Peptone Yeast Extract Broth (PY)         10 tubes / pkg $17.24
AS-834 Peptone Yeast Extract Broth with Adonitol (PY ADONITOL)        10 tubes / pkg $17.57
AS-836 Peptone Yeast Extract Broth with Amygdalin (PY AMYGDALIN)         10 tubes / pkg $38.83
AS-824 Peptone Yeast Extract Broth with Arabinose (PY ARABINOSE)         10 tubes / pkg $17.57
AS-835 Peptone Yeast Extract Broth with Arginine (PY ARGININE)        10 tubes / pkg $17.57
AS-857 Peptone Yeast Extract Broth with Glucose & Bile (PYG BILE)         10 tubes / pkg $17.57
AS-837 Peptone Yeast Extract Broth with Cellobiose (PY CELLOBIOSE)         10 tubes / pkg $17.57
AS-838 Peptone Yeast Extract Broth with Dulcitol (PY DULCITOL)         10 tubes / pkg $17.57
AS-839 Peptone Yeast Extract Broth with Erythritol (PY ERYTHRITOL)         10 tubes / pkg $17.57
AS-833 Peptone Yeast Extract Broth with Esculin (PY ESCULIN)         10 tubes / pkg $17.57
AS-840 Peptone Yeast Extract Broth with Fructose (PY FRUCTOSE)         10 tubes / pkg $17.57
AS-841 Peptone Yeast Extract Broth with Galactose (PY GALACTOSE)         10 tubes / pkg $17.57
AS-860 Peptone Yeast Extract Broth with Glucose & Gelatin (PYG GELATIN)         10 tubes / pkg $17.57
AS-822 Peptone Yeast Extract Broth with Glucose (PYG)         10 tubes / pkg $17.24
AS-842 Peptone Yeast Extract Broth with Glycerol (PY GLYCEROL)         10 tubes / pkg $17.57
AS-843 Peptone Yeast Extract Broth with Glycogen (PY GLYCOGEN)              10 tubes / pkg $17.57
AS-844 Peptone Yeast Extract Broth with Inositol (PY INOSITOL)         10 tubes / pkg $17.57
AS-845 Peptone Yeast Extract Broth with Inulin (PY INULIN)         10 tubes / pkg $17.57
AS-826 Peptone Yeast Extract Broth with Lactose (PY LACTOSE)         10 tubes / pkg $17.57
AS-847 Peptone Yeast Extract Broth with Maltose (PY MALTOSE)         10 tubes / pkg $17.57
AS-848 Peptone Yeast Extract Broth with Mannitol (PY MANNITOL)         10 tubes / pkg $17.57
AS-849 Peptone Yeast Extract Broth with Mannose (PY MANNOSE)         10 tubes / pkg $17.57
AS-850 Peptone Yeast Extract Broth with Melezitose (PY MELEZITOSE)         10 tubes / pkg $17.57
AS-851 Peptone Yeast Extract Broth with Melibiose (PY MELIBIOSE)         10 tubes / pkg $17.57
AS-853 Peptone Yeast Extract Broth with Raffinose (PY RAFFINOSE)         10 tubes / pkg $17.57
AS-827 Peptone Yeast Extract Broth with Rhamnose (PY RHAMNOSE)         10 tubes / pkg $19.85
AS-854 Peptone Yeast Extract Broth with Ribose (PY RIBOSE)         10 tubes / pkg $17.57
AS-828 Peptone Yeast Extract Broth with Salicin (PY SALICIN)         10 tubes / pkg $19.85
AS-855 Peptone Yeast Extract Broth with Sorbitol (PY SORBITOL)         10 tubes / pkg $17.57
AS-829 Peptone Yeast Extract Broth with Starch (PY STARCH)         10 tubes / pkg $17.24
AS-830 Peptone Yeast Extract Broth with Sucrose (PY SUCROSE)         10 tubes / pkg $17.57
AS-831 Peptone Yeast Extract Broth with Trehalose (PY TREHALOSE)         10 tubes / pkg $17.57
AS-859 Peptone Yeast Extract Broth with Xylan (PY XYLAN)         10 tubes / pkg $17.57
AS-832 Peptone Yeast Extract Broth with Xylose (PY XYLOSE)         10 tubes / pkg $17.57
PY SUGARS InsertPY SDS

Product Description & Summary

Peptone Yeast Extract Broth Based Media are enriched nonselective media that are supplemented with hemin and vitamin K1 to facilitate the recovery of the more fastidious organisms such as Prevotella spp, Porphyromonas spp, and the Bacteroides fragilis group. Resazurin is added as a redox indicator. PRAS (Pre-reduced anaerobically sterilized) biochemicals are considered to be the “gold standard” for biochemical testing and characterization of anaerobes. With the addition of a specific biochemical, the Peptone Yeast Extract Broth Based Media are used to determine whether anaerobic bacteria will utilize the biochemical or if growth is stimulated or inhibited. PY and PYG are nonselective broth media originally formulated by the VPI group for use in chromatographic analysis of metabolic products from the fermentation of glucose. This chromatographic analysis is useful in the identification of clinically important anaerobic bacteria. This media is prepared, dispensed, and packaged under oxygen-free conditions to prevent the formation of oxidized products prior to use.

Interpretation of Results & Limitations

If used properly, fermentation is indicated when the pH of the inoculated media is lowered after incubation. Un-inoculated Peptone Yeast Extract Broth Based Media should show only trace amounts, if any, of volatile and nonvolatile fatty acids when tested with gas liquid chromatography. PYG cultures of appropriate control strains, such as Bacteroides fragilis, Fusobacterium necrophorum, and Clostridium perfringens, should have characteristic metabolic products when tested with gas chromatography. Peptone Yeast Extract Broth Based Media should support good growth of anaerobes isolated from clinical specimens.

pH Measurements: Once sufficient growth has been observed, the pH can be read directly from the culture tube using a pH meter and a long thin combination electrode. Thoroughly rinse the electrode with distilled water into a container containing disinfectant after reading each strain. Disinfect the electrode before returning it to the storage solution.

Interpretation of pH: A pH value of 5.5 to 6.0 is considered a weak acid production, while a value of 5.5 or less is a strong acid production. A pH above 6.0 is considered no acid production.

Esculin Hydrolysis Detection (AS-833 PY ESCULIN): Add 2 to 3 drops of a 1% solution of ferric ammonium citrate to an inoculated tube of PY ESCULIN with sufficient bacterial growth. Blackening of the medium indicates that the esculin present has been hydrolyzed by the organism.

Starch Hydrolysis Detection (AS-829 PY STARCH): Add 2 to 3 drops of diluted 1:5 of Gram’s Iodine to an inoculated tube of PY STARCH with sufficient growth. No color change indicates hydrolysis of starch by the organism. The formation of blue/black color indicates that starch is present.

Gelatin Hydrolysis Detection (AS-860 PYG GELATIN): Place a 24 – 72 hour culture with an uninoculated control tube in a 2 – 8 °C environment. The uninoculated control tube will solidify, and, if the inoculated tube does not solidify, then the gelatin has been hydrolyzed. Solidification of the inoculated tube results in no gelatin hydrolysis.

Growth in Bile Determination (AS-857 PYG BILE): Turbidity in the medium is compared to that of PYG (AS-822) to determine if the organism is inhibited or stimulated by bile.

Peptone Yeast Extract Broth Based Media will not provide complete information for identification of bacterial isolates. Additional test procedures and media are required for complete identification. Consult reference materials for additional information.

Quality Control

The following organisms are routinely used at Anaerobe Systems for quality assurance performance testing for basic growth of Peptone Yeast Extract Broth Based Media.

Organism Tested ATCC # Results Time
Bacteroides vulgatus 8482 Growth 24 hrs
Bacteroides fragilis 25285 Growth 24 hrs
Prevotella melaninogenica 25845 Growth* 24 – 48 hrs
Fusobacterium necrophorum 25286 Growth* 24 – 48 hrs
Fusobacterium nucleatum 25586 Growth* 24 – 48 hrs
Clostridium perfringens 13124 Growth 24 hrs
Peptostreptococcus anaerobius 27337 Growth* 24 hrs
Clostridium novyi, Type A          (PY and PYG only) 7659 Growth 48 – 72 hrs
Propionibacterium acnes           (PY, PY ADONITOL, PYG, and PY GLYCEROL only) 6919 Growth 24 – 48 hrs
Staphylococcus aureus               (PY and PYG only) 25923 Growth 24 hrs
Peptoniphilus asaccharolyticus (PY FRUCTOSE, PYG GELATIN, PYG BILE, and PYG only) 29743 Growth* 24 – 48 hrs

*No growth in PYG BILE

Product Storage & Shelf Life

Storage: Upon receipt, store at room temperature in original package until used. Avoid overheating or freezing. Do not use media if there are signs of deterioration (discoloration due to oxidation of media) or contamination. The expiration date applies to the product in its original packaging and stored as directed. Do not use product past the expiration date shown on the label.

Shelf Life: 1 year from date of manufacture.