PRAS Dilution Blanks

PRAS Dilution Blanks

Dilution Blanks are a buffered mineral salt base solution with reducing agents, designed as a holding medium for maintaining viability of microorganisms, especially anaerobes, in order to determine the number of viable organisms present in a sample.

Dilution Blanks

Dilution Blanks Packaging

Catalog #
AS-907 Dilution Blank – 7 mL 10 Tubes / pkg $19.95
AS-908 Dilution Blank – 9 mL 10 Tubes / pkg $14.76
AS-910 Dilution Blank – 10 mL 10 Tubes / pkg $14.76
AS-917 Dilution Blank – 3 mL 10 Tubes / pkg $19.95
AS-918 Dilution Blank – 18 mL 10 Tubes / pkg $24.95

Product Description & Summary

Bacterial cells are often diluted from their original dense concentration to a sparse concentration to become suitable for use in microscopy, quantitation, genetic analysis, or metabolic studies. Dilution Blanks are a buffered mineral salt solution with sodium thioglycollate and L-cysteine added to provide a reduced environment. This formulation has been prepared to provide an environment which maintains the viability of most microorganisms without significant multiplication. Dilution Blanks have been designed to meet the stringent viability requirements of obligate anaerobes. This medium is prepared, dispensed, and packaged under oxygen-free conditions to prevent the formation of oxidized products prior to use.

Interpretation of Results & Limitations

If properly used, Dilution Blanks will maintain the viability of microorganisms within clinical material in order to determine the number of viable organisms present in the sample. Organisms are serially diluted and recovered on BRU agar (AS-111).

Dilution Blanks are designed to be a holding medium to maintain the viability of microorganisms contained in a specimen. Other media will be required for isolation and identification of clinical isolates. Specimens should be transported to the laboratory and processed as soon as possible, as delayed processing may result in overgrowth by one organism in a specimen from polymicrobial infections. Consult reference materials for additional information.

Quality Control

The following organisms are routinely used for quality control testing at Anaerobe Systems.

Organism Tested ATCC # Results Time
Bacteroides fragilis 25285 Growth 24 hrs
Prevotella melaninogenica 25845 Growth 24 – 48 hrs

Product Storage & Shelf Life

Storage: Upon receipt, store at room temperature in original package until used. Avoid overheating or freezing. Do not use media if there are signs of deterioration (shrinking, cracking, or discoloration due to oxidation of media) or contamination. The expiration date applies to the product in its original packaging and stored as directed. Do not use product past the expiration date shown on the label.

Shelf Life: 1 year from date of manufacture.