Peptone Yeast Extract Broth with Glucose and Formate/Fumarate FA/GLC (PYG FF FA/GLC) is designed for the determination of glucose fermentation and formate/fumarate stimulation by anaerobic bacteria for identification. PYG FF FA/GLC is recommended for use in identifying Campylobacter ureolyticus within the MIDI MIS procedures.
Peptone Yeast Extract Broth with Glucose and Formate/Fumarate FA/GLC (PYG FF FA/GLC)
Product Description & Summary
PYG FF FA/GLC is a non-selective broth medium that was formulated by the VPI group for use in chromatographic analysis of cellular fatty acids and short chain fatty acid end products from the fermentation of glucose and utilization/stimulation by formate/fumarate. This analysis is useful in the identification of clinically significant anaerobic bacteria, like Campylobacter ureolyticus. This media is prepared, dispensed, and packaged under oxygen-free conditions to prevent the formation of oxidized products prior to use.
Interpretation of Results & Limitations
Uninoculated PY based broths should show only trace amounts, if any, of volatile and nonvolatile fatty acids when tested with gas liquid chromatography. If used properly, this medium, when cultured with appropriate control strains, will show characteristic metabolic products when analyzed using MIDI MIS system. For interpretation of chromatographic results, consult the MIDI MIS manual.
The following organisms are routinely used for quality assurance performance testing, for growth and the fermentation of glucose, at Anaerobe Systems. Formate/Fumarate utilization is determined on the basis of growth stimulation when compared to Peptone Yeast Extract Broth w/ Glucose (PYG).
|Organism Tested||ATCC #
|Bacteroides fragilis*||25285||Growth||24 hrs|
|Prevotella melaninogenica||25845||Growth||24 – 48 hrs|
|Bacteroides vulgatus||8482||Growth||24 hrs|
|Fusobacterium nucleatum||25586||Growth||24 – 48 hrs|
|Fusobacterium necrophorum||25286||Growth||24 – 48 hrs|
|Clostridium perfringens||13124||Growth||24 hrs|
|Peptostreptococcus anaerobius||27337||Growth||24 hrs|
|Peptoniphilus asaccharolyticus||29743||Growth||24 – 48 hrs|
|Campylobacter ureolyticus*||33387||Growth||24 – 48 hrs||+|
*Organisms recommended by MIDI MIS manual for quality control.
Product Storage & Shelf Life
Storage: Upon receipt, store at room temperature in original package until used. Avoid overheating or freezing. Do not use media if there are signs of deterioration (shrinking, cracking, or discoloration due to oxidation of media) or contamination. The expiration date applies to the product in its original packaging and stored as directed. Do not use product past the expiration date shown on the label.
Shelf Life: 1 year from date of manufacture.