PYG Tween for FA/GLC

PEPTONE YEAST EXTRACT BROTH WITH GLUCOSE AND TWEEN 80 FA/GLC
(PYG TWEEN FA/GLC)

Peptone Yeast Extract Broth with Glucose and Tween 80 FA/GLC (PYG TWEEN FA/GLC) is designed for the determination of glucose fermentation and Tween 80 stimulation by anaerobic bacteria for identification. PYG FA/GLC is recommended for use in identifying gram-positive bacteria within the MIDI MIS procedures.

PYG Tween Broth for FA/GLC

PYG Tween Broth for FA/GLC Packaging

Catalog #
Description
Qty
Price
AS-825 PYG Tween for FA/GLC 4 plates $20.01
PYG TWEEN Product InsertPYG TWEEN SDS

Product Description & Summary

Peptone Yeast Extract Broth with Glucose and Tween 80 FA/GLC (PYG TWEEN FA/GLC) is designed for the determination of glucose fermentation and Tween 80 stimulation by anaerobic bacteria for identification. PYG FA/GLC is recommended for use in identifying gram-positive bacteria within the MIDI MIS procedures.

Interpretation of Results & Limitations

Uninoculated PY based broths should show only trace amounts, if any, of volatile and nonvolatile fatty acids when tested with gas liquid chromatography. If used properly, this medium, when cultured with appropriate control strains, will show characteristic metabolic products when analyzed using MIDI MIS system. For interpretation of chromatographic results, consult the MIDI MIS manual.

PYG TWEEN FA/GLC will not provide complete information for identification of bacterial isolates. Additional test procedures and media are required for complete identification. Consult reference materials for additional information.

Quality Control

The following organisms are routinely used for quality assurance performance testing, for growth and the fermentation of glucose, at Anaerobe Systems. Glucose utilization is determined on the basis of pH with the production of acid. A strong acid will result in a pH of 5.5 or below, weak acid results in a pH of 5.5 – 6.0, and no acid production results in a pH of 6.0 or above. Tween 80 growth stimulation is determined on comparison of growth against a control medium, like Peptone Yeast Extract Broth with Glucose (AS-822).

 

Organism Tested ATCC # Results Time Glucose Utilization
Bacteroides fragilis 25285 Growth 24 hrs NT
Prevotella melaninogenica 25845 Growth 24 – 48 hrs NT
Bacteroides vulgatus 8482 Growth 24 hrs NT
Fusobacterium nucleatum 25586 Growth 24 – 48 hrs NT
Fusobacterium necrophorum 25286 Growth 24 – 48 hrs NT
Clostridium perfringens* 13124 Growth 24 hrs +
Peptostreptococcus anaerobius 27337 Growth 24 hrs NT
Peptoniphilus asaccharolyticus 29743 Growth 24 – 48 hrs
Clostridium novyi t 6919 Growth 24 – 48 hrs +

*This organism is tested for characteristic fatty acid results using the MIDI MIS system.               NT = Not Tested

t Growth stimulated when compared to Peptone Yeast Extract Broth with Glucose (PYG; AS-822).

Product Storage & Shelf Life

Storage: Upon receipt, store at room temperature in original package until used. Avoid overheating or freezing. Do not use media if there are signs of deterioration (shrinking, cracking, or discoloration due to oxidation of media) or contamination. The expiration date applies to the product in its original packaging and stored as directed. Do not use product past the expiration date shown on the label.

Shelf Life: 1 year from date of manufacture.