Peptone Yeast Extract Broth with Glucose and Tween 80 (PYG TWEEN) is designed for the determination of glucose fermentation and Tween 80 stimulation by anaerobic bacteria for identification.
PEPTONE YEAST EXTRACT BROTH WITH GLUCOSE AND TWEEN 80
(PYG TWEEN)
PYG Tween Broth
PYG Tween Broth Packaging
| Catalog # |
Description |
Qty |
Price |
| AS-825 | PYG Tween | 10 tubes | $32.39 |
Non-stock item: 15 pack minimum order, allow 2-4 weeks for delivery.
Product Description & Summary
Peptone Yeast Extract Broth with Glucose and Tween 80 (PYG TWEEN) is designed for the determination of glucose fermentation and Tween 80 stimulation by anaerobic bacteria for identification.
Interpretation of Results & Limitations
Uninoculated PY based broths should show only trace amounts, if any, of volatile and nonvolatile fatty acids when tested with gas liquid chromatography.
PYG TWEEN will not provide complete information for identification of bacterial isolates. Additional test procedures and media are required for complete identification. Consult reference materials for additional information.
Quality Control
The following organisms are routinely used for quality assurance performance testing, for growth and the fermentation of glucose, at Anaerobe Systems. Glucose utilization is determined on the basis of pH with the production of acid. A strong acid will result in a pH of 5.5 or below, weak acid results in a pH of 5.5 – 6.0, and no acid production results in a pH of 6.0 or above. Tween 80 growth stimulation is determined on comparison of growth against a control medium, like Peptone Yeast Extract Broth with Glucose (AS-822).
| Organism Tested | ATCC # | Results | Time | |
| Bacteroides fragilis | 25285 | Growth | 24 hrs | |
| Prevotella melaninogenica | 25845 | Growth | 24 – 48 hrs | |
| Bacteroides vulgatus | 8482 | Growth | 24 hrs | |
| Fusobacterium nucleatum | 25586 | Growth | 24 – 48 hrs | |
| Fusobacterium necrophorum | 25286 | Growth | 24 – 48 hrs | |
| Clostridium perfringens | 13124 | Growth | 24 hrs | |
| Peptostreptococcus anaerobius | 27337 | Growth | 24 hrs | |
| Peptoniphilus asaccharolyticus | 29743 | Growth | 24 – 48 hrs | |
| Clostridium novyi t | 6919 | Growth | 24 – 48 hrs |
t Growth stimulated when compared to Peptone Yeast Extract Broth with Glucose (PYG; AS-822).
Product Storage & Shelf Life
Storage: Upon receipt, store at room temperature in original package until used. Avoid overheating or freezing. Do not use media if there are signs of deterioration (shrinking, cracking, or discoloration due to oxidation of media) or contamination. The expiration date applies to the product in its original packaging and stored as directed. Do not use product past the expiration date shown on the label.
Shelf Life: 1 year from date of manufacture.