Reinforced Clostridial Broth – RCB

Reinforced Clostridial Broth – RCB

Reinforced Clostridial Broth (RCB) is an enriched non-selective medium intended for the cultivation of Clostridia and other anaerobic and facultative bacteria from various clinical and non-clinical specimens.

RCB Broth

RCB Packaging

Large Format Options

Catalog #
AS-606 Reinforced Clostridial Broth, 7mL – RCB 10 tubes / pkg $23.88
AS-6062 RCB Broth, 250mL 1 Bottle
AS-6065 RCB Broth, 500mL 1 Bottle

Non-stock items:
AS-606 – 15 pack minimum order, allow 2-4 weeks for delivery.
AS-6062 – 10 bottle minimum order, allow 2-4 weeks for delivery.
AS-6065 – 5 bottle minimum order, allow 2-4 weeks for delivery.


Product Description & Summary

RCB is a enriched non-selective broth medium formulated by Hirsch and Grinstead to enhance the growth of clostridia from a small inoculum. Casein, beef extract, and yeast extract form the nutritive base that supplies a source of carbon, nitrogen, vitamins, and minerals. Dextrose is added as a source of carbohydrates. L-Cysteine is included as a reducing agent. Soluble starch is a detoxifier of metabolic by-products while sodium acetate acts as a buffer. Resazurin is added as an indicator of significant oxygen exposure to the media. This media is prepared, dispensed, and packaged under oxygen-free conditions to prevent the formation of oxidized products prior to use.

Interpretation of Results & Limitations

If used properly, RCB will support the good growth of Clostridia and many other anaerobes isolated from clinical or non-clinical specimens.

RCB will not provide complete information for identification of bacterial isolates. Additional test procedures and media are required for complete identification. Consult reference materials for additional information.

Quality Control

The following organisms are routinely used for quality control testing at Anaerobe Systems.

Organism Tested ATCC #


Results Time
Bacteroides fragilis 25285 Growth 24 hrs
Propionibacterium acnes 6919 Growth 24 – 48 hrs
Enterococcus faecalis 29212 Growth 24 hrs
Clostridium difficile 9689 Growth 24 hrs
Clostridium difficile 700057 Growth 24 hrs
Clostridium perfringens 13124 Growth 24 hrs
Clostridium innocuum 14501 Growth 24 hrs
Clostridium sporogenes 3584 Growth 24 hrs
Clostridium beijerinckii 8260 Growth 24 hrs
Clostridium sordellii 9714 Growth 24 hrs

Product Storage & Shelf Life

Storage: Upon receipt, store at room temperature in original package until used. Avoid overheating or freezing. Do not use media if there are signs of deterioration (discoloration due to oxidation of media) or contamination. The expiration date applies to the product in its original packaging and stored as directed. Do not use product past the expiration date shown on the label.

Shelf Life: 1 year from date of manufacture.