Thioglycollate Broth (THIO) is an enriched nonselective liquid medium used for the growth of microaerophilic and anaerobic bacteria, including fastidious organisms, from clinical specimens.
Thioglycollate Broth with Hemin, Vit. K, without indicator – THIO
Product Description & Summary
THIO is an enriched nonselective liquid medium used for the growth of microaerophilic and fastidious anaerobic organisms. THIO contain casein, meat peptones, yeast extract, and dextrose as the nutritive base. Sodium thioglycollate and L-cystine are included as reducing agents. Hemin and vitamin K1 are included to promote the growth of fastidious anaerobes. A small quantity of agar is added to slow the diffusion of oxygen. A marble chip is included to each tube to act as a pH buffer and neutralize acids produced during growth of the organisms. This media is prepared, dispensed, and packaged under oxygen-free conditions to prevent the formation of oxidized products prior to use.
Interpretation of Results & Limitations
If used properly, THIO will support good growth of many commonly isolated anaerobes from clinical specimens, including known strains of Clostridium perfringens, Bacteroides fragilis, Prevotella melaninogenica, and Fusobacterium necrophorum.
THIO will not provide complete information for identification of bacterial isolates. Additional test procedures and media are required for complete identification. Consult reference materials for additional information.
The following organisms are routinely used for quality control testing at Anaerobe Systems.
|Organism Tested||ATCC #||Results||Time|
|Bacteroides fragilis*||25285||Growth||24 hrs|
|Prevotella melaninogenica||25845||Growth||24 – 48 hrs|
|Bacteroides vulgatus*||8482||Growth||24 hrs|
|Fusobacterium necrophorum||25286||Growth||24 – 48 hrs|
|Clostridium perfringens*||13124||Growth||24 hrs|
|Clostridium novyi||7659||Growth||48 hrs|
|Peptostreptococcus anaerobius*||27337||Growth||24 hrs|
|Staphylococcus aureus*||25923||Growth||24 hrs|
* Organisms specified by CLSI for quality control testing of Thioglycollate Broth.
Product Storage & Shelf Life
Storage: Upon receipt, store at room temperature in original package until used. Avoid overheating or freezing. Do not use media if there are signs of deterioration (shrinking, cracking, or discoloration due to oxidation of media) or contamination. The expiration date applies to the product in its original packaging and stored as directed. Do not use product past the expiration date shown on the label.
Shelf Life: 1 year from date of manufacture.