CDC is a tryptic soy agar base supplemented with vitamin K1 and hemin to facilitate the recovery of more fastidious organisms, such as Prevotella, Porphyromonas, and the Bacteroides fragilis group, and should facilitate the pigment production of Prevotella melaninogenica. Sheep blood is added for the observation of hemolytic reactions as seen by the double zone of β-hemolysis of Clostridium perfringens, and various growth factors. This media is prepared, dispensed, and packaged under oxygen-free conditions to prevent the formation of oxidized products prior to use.
Anaerobe Blood Agar – CDC
CDC Agar Plate
C. perfringens (ATCC 13124) Growing On CDC Agar Plate
4 plates per package
| Catalog # |
Description |
Qty |
Price |
| AS-646 | CDC Anaerobic Blood Agar (CDC) | 4 plates |
$11.60 |
Product Description & Summary
CDC is a tryptic soy agar base supplemented with vitamin K1 and hemin to facilitate the recovery of more fastidious organisms, such as Prevotella, Porphyromonas, and the Bacteroides fragilis group, and should facilitate the pigment production of Prevotella melaninogenica. Sheep blood is added for the observation of hemolytic reactions as seen by the double zone of β-hemolysis of Clostridium perfringens, and various growth factors. This media is prepared, dispensed, and packaged under oxygen-free conditions to prevent the formation of oxidized products prior to use.
Interpretation of Results & Limitations
If used properly, CDC supports the growth of obligate anaerobes found in clinical infections. In addition, the media should support typical pigment production by pigmented Prevotella and Porphyromonas spp. The media should support typical double zone of β-hemolysis around colonies of Clostridium perfringens.
CDC Anaerobic Blood Agar will not provide complete information for identification of bacterial isolates. Additional test procedures and media are required for complete identification. It is recommended that a selective media such as Anaerobic Brucella Laked Blood Agar with Kanamycin and Vancomycin (LKV, catalog#: AS-112) and/or Anaerobic Brucella Blood Agar with Phenylethyl Alcohol (PEA, catalog#: AS-113) also be inoculated with clinical specimens to assure growth of all species present. Consult reference materials for additional information.
Quality Control
The following organisms are routinely used for quality assurance performance testing at Anaerobe Systems.
| Organism Tested | ATCC # | Results | Time | Special Reaction |
| Bacteroides fragilis * | 25285 | Growth | 24 hrs | |
| Prevotella melaninogenica * | 25845 | Growth | 24-48 hrs | Pigmentt (tan to brown) |
| Fusobacterium necrophorum | 25286 | Growth | 24 hrs | |
| Fusobacterium nucleatum * | 25586 | Growth | 24 hrs | |
| Clostridium perfringens * | 13124 | Growth | 24 hrs | Double zone β-hemolysis |
| Peptostreptococcus anaerobius * | 27337 | Growth | 24 hrs | |
| Staphylococcus aureus or Enterococcus faecalis | 25923 29212 | Growth
Growth |
24 hrs
24 hrs |
|
| Escherichia coli | 25922 | Growth | 24 hrs | |
| Proteus mirabilis | 12453 | Growth | 24 hrs | |
| Clostridium difficile or Propionibacterium acnes | 9689 6919 | Growth
Growth |
24 hrs
24-48 hrs |
*Organisms specified by CLSI for Quality Control testing for Anaerobic Blood Agars
t Pigment production may require more than 48 hours of incubation.
Product Storage & Shelf Life
Storage: Upon receipt, store at room temperature in original package until used. Avoid overheating or freezing. Do not use media if there are signs of deterioration (shrinking, cracking, or discoloration due to oxidation of media) or contamination. The expiration date applies to the product in its original packaging and stored as directed. Do not use product past the expiration date shown on the label.
Shelf Life: 90 days from date of manufacture.