Anaerobe Blood Agar – CDC

Anaerobe Blood Agar – CDC

CDC is a tryptic soy agar base supplemented with vitamin K1 and hemin to facilitate the recovery of more fastidious organisms, such as Prevotella, Porphyromonas, and the Bacteroides fragilis group, and should facilitate the pigment production of Prevotella melaninogenica. Sheep blood is added for the observation of hemolytic reactions as seen by the double zone of β-hemolysis of Clostridium perfringens, and various growth factors. This media is prepared, dispensed, and packaged under oxygen-free conditions to prevent the formation of oxidized products prior to use.

CDC Agar Plate

C. perfringens (ATCC 13124) Growing On CDC Agar Plate

4 plates per package

Catalog #
Description
Qty
Price
AS-646 CDC Anaerobic Blood Agar (CDC) 4 plates
$9.18
CDC Product InsertCDC SDS

Product Description & Summary

CDC is a tryptic soy agar base supplemented with vitamin K1 and hemin to facilitate the recovery of more fastidious organisms, such as Prevotella, Porphyromonas, and the Bacteroides fragilis group, and should facilitate the pigment production of Prevotella melaninogenica. Sheep blood is added for the observation of hemolytic reactions as seen by the double zone of β-hemolysis of Clostridium perfringens, and various growth factors. This media is prepared, dispensed, and packaged under oxygen-free conditions to prevent the formation of oxidized products prior to use.

Interpretation of Results & Limitations

If used properly, CDC supports the growth of obligate anaerobes found in clinical infections. In addition, the media should support typical pigment production by pigmented Prevotella and Porphyromonas spp. The media should support typical double zone of β-hemolysis around colonies of Clostridium perfringens.

CDC Anaerobic Blood Agar will not provide complete information for identification of bacterial isolates. Additional test procedures and media are required for complete identification. It is recommended that a selective media such as Anaerobic Brucella Laked Blood Agar with Kanamycin and Vancomycin (LKV, catalog#: AS-112) and/or Anaerobic Brucella Blood Agar with Phenylethyl Alcohol (PEA, catalog#: AS-113) also be inoculated with clinical specimens to assure growth of all species present. Consult reference materials for additional information.

Quality Control

The following organisms are routinely used for quality assurance performance testing at Anaerobe Systems.

Organism Tested ATCC # Results Time Special Reaction
Bacteroides fragilis * 25285 Growth 24 hrs
Prevotella melaninogenica * 25845 Growth 24-48 hrs Pigmentt (tan to brown)
Fusobacterium necrophorum 25286 Growth 24 hrs
Fusobacterium nucleatum * 25586 Growth 24 hrs
Clostridium perfringens * 13124 Growth 24 hrs Double zone β-hemolysis
Peptostreptococcus anaerobius * 27337 Growth 24 hrs
Staphylococcus aureus or Enterococcus faecalis 25923       29212 Growth

Growth

24 hrs

24 hrs

Escherichia coli 25922 Growth 24 hrs
Proteus mirabilis 12453 Growth 24 hrs
Clostridium difficile or Propionibacterium acnes 9689       6919 Growth

Growth

24 hrs

24-48 hrs

*Organisms specified by CLSI for Quality Control testing for Anaerobic Blood Agars
t Pigment production may require more than 48 hours of incubation.

Product Storage & Shelf Life

Storage: Upon receipt, store at room temperature in original package until used. Avoid overheating or freezing. Do not use media if there are signs of deterioration (shrinking, cracking, or discoloration due to oxidation of media) or contamination. The expiration date applies to the product in its original packaging and stored as directed. Do not use product past the expiration date shown on the label.

Shelf Life: 90 days from date of manufacture.