Bifidobacterium Selective Agar – BIFIDO

Bifidobacterium Selective Agar – BIFIDO

Bifidobacterium Selective Agar (BIFIDO) is a selective media for the isolation and enumeration of Bifidobacterium species.

BIFIDO Agar Plate

B. adolescentis (ATCC 15703) Growing On BIFIDO Agar Plate

4 plates per package

Catalog #
Qty Price
AS-6423 Bifidobacterium Selective Agar (BIFIDO) 4 plates $13.16

Non-stock item: 25 pack minimum order, allow 2-4 weeks for delivery.


Product Description & Summary

BIFIDO agar contains a reinforced clostridial agar formulation as the basal nutritive media. Selective and differential compounds include iodoacetic acid and 2, 3, 5-triphenyltetrazolium chloride (TTC). Iodoacetate inhibits glyceraldehyde-3-phosphate dehydrogenase, drastically reducing the growth of non-bifidobacterial colonies. TTC makes it possible to differentiate between other species and Bifidobacterium, since the bifidobacterial develop in large white colonies. This media is prepared, dispensed, and packaged under oxygen-free conditions to prevent the formation of oxidized products prior to use.

Interpretation of Results & Limitations

This media supports good growth of some of the Bifidobacterium spp. found in clinical infections.

BIFIDO will not provide complete information for identification of bacterial isolates. Additional test procedures and media are required for complete identification. It is recommended that a non-selective media, such as Brucella Blood Agar (BRU, catalog #: AS-111) also be inoculated from the same clinical specimen to assure recovery of all species present. Consult reference materials for additional information.

Quality Control

The following organisms are routinely used for quality control testing at Anaerobe Systems.

Organism Tested ATCC # Results Time
Bacteroides fragilis 25285 Growth 24 – 48 hrs
Fusobacterium nucleatum 25586 No Growth
Clostridium perfringens 13124 Inhibited
Peptostreptococcus anaerobius 27337 Variable
Proteus mirabilis 12453 Inhibited
Staphylococcus aureus 25923 Variable
Escherichia coli 25922 No Growth
Bifidobacterium longum 15707 Growth 48 – 72 hrs
Bifidobacterium bifidum 15696 Growth 48 – 72 hrs
Bifidobacterium adolescentis 15703 Growth 48 – 72 hrs
Bifidobacterium breve 15700 Variable 48 – 72 hrs


Product Storage & Shelf Life

Storage: Upon receipt, store at room temperature in original package until used. Avoid overheating or freezing. Do not use media if there are signs of deterioration (shrinking, cracking, or discoloration due to oxidation of media) or contamination. The expiration date applies to the product in its original packaging and stored as directed. Do not use product past the expiration date shown on the label.

Shelf Life: 90 days from date of manufacture.