Botulism Selective Media – BSM

Botulism Selective Media – BSM

Botulism Selective Medium (BSM) is a selective and differential medium for the presumptive identification of Clostridium botulinum from food or clinical samples.

BSM Agar Plate

C. sporogenes (ATCC 3584) Growing On BSM Agar Plate

4 plates per package

Catalog #
Description
Qty
Price
AS-223 BSM mono plate 4 plates
$14.06

Non-stock item: 20 pack minimum order, allow 2-4 weeks for delivery.

BSM Product InsertBSM SDS

Product Description & Summary

BSM agar is a selective and differential media used for the presumptive identification of Clostridium botulinum from food or fecal samples. BSM contains cycloserine, sulfamethoxazole, and trimethoprim as selective inhibitory agents. A suspension of egg yolk is added for the detection of lecithinase, lipase, and proteolytic activity. The degradation of lecithin in the egg yolk results in an opaque precipitate around the colonies. Lipase destroys the fats within the egg yolk, which results in an iridescent sheen on the colony surface. Proteolytic activity is indicated by a clearing of the medium around the colonies. This medium is dispensed under oxygen-free conditions to prevent the formation of oxidized products prior to use.

Interpretation of Results & Limitations

BSM supports good growth of Clostridium botulinum from food or clinical infections. This medium supports the typical lecithinase and lipase reactions produced by some Clostridia species. Degradation of lecithin in the egg yolk, from lecithinase positive organisms, results in an opaque precipitate surrounding the colonies. Destruction of the fats within the egg yolk, from lipase positive organisms, results in an iridescent sheen on the colony surface.

BSM will not provide complete information for identification of bacterial isolates. Additional test procedures and media are required for complete identification. It is recommended that a non-selective media, such as Brucella Blood Agar (BRU, Cat. #: AS-111) also be inoculated from the same clinical specimen to assure recovery of all species present. Consult reference materials for additional information.

Quality Control

The following organisms are routinely used for quality assurance testing at Anaerobe Systems.

Organism Tested ATCC # Results Time Special Reaction
Bacteroides fragilis 25285 No Growth
Prevotella melaninogenica 25845 Inhibited
Fusobacterium necrophorum 25286 No Growth
Fusobacterium nucleatum 25586 No Growth
Clostridium perfringens 13124 Inhibited Lecithinase reaction
Clostridium difficile 9689 Inhibited
Clostridium novyi 7659 No Growth
Clostridium sporogenes 3584 Growth 24 – 48 hours Lipase reaction
Propionibacterium acnes 6919 No Growth
Peptostreptococcus anaerobius 28337 No Growth
Escherichia coli 25922 No Growth
Staphylococcus aureus 25923 No Growth

Product Storage & Shelf Life

Storage: Upon receipt, store at room temperature in original package until used. Avoid overheating or freezing. Do not use media if there are signs of deterioration (shrinking, cracking, or discoloration due to oxidation of media) or contamination. The expiration date applies to the product in its original packaging and stored as directed. Do not use product past the expiration date shown on the label.

Shelf Life: 90 days from date of manufacture.