Brain Heart Infusion Agar w/ Horse Blood & Taurocholate – BHIY-HT

Brain Heart Infusion Agar w/ Horse Blood & Taurocholate – BHIY-HT

Brain Heart Infusion Agar with Horse Blood and Taurocholate (BHIY-HT) is an enriched non-selective media for the germination and cultivation of Clostridium difficile cells and spores along with other fastidious anaerobic microorganisms.

BHIY-HT Agar Plate

C. difficile (ATCC 700057) Growing On BHIY-HT Agar Plate

4 plates per package

Catalog #
AS-6463 BHIY-HT mono plate 4 plates

Product Description & Summary

BHIY-HT agar is an enriched non-selective media used for the isolation and cultivation of a wide variety of microorganisms, including yeast and molds. The basic nutritive properties are brain heart infusion, meat peptone, and yeast extract. Horse blood is added to stimulate growth. Sodium taurocholate is added to promote the germination of Clostridium difficile spores. When grown on this media, Clostridium difficile will exhibit characteristic colonial morphology, fluorescence, and smell. This media is prepared, dispensed, and packaged under oxygen-free conditions to prevent the formation of oxidized products prior to use.

Interpretation of Results & Limitations

This media supports good growth of many fastidious and non-fastidious anaerobes isolated from clinical specimens.

BHIY-HT will not provide complete information for identification of bacterial isolates. Additional test procedures and media are required for complete identification. Consult reference materials for additional information.

Quality Control

The following organisms are routinely used for quality control testing at Anaerobe Systems.

Organism Tested ATCC # Results Time
Bacteroides fragilis 25285 Growth 24 hrs
Enterococcus faecalis 29212 Growth 24 hrs
Clostridium sporogenes 3584 Growth 24 hrs
Clostridium beijerinckii 8260 Inhibited 24 hrs
Proteus mirabilis 12453 Growth 24 hrs
Clostridium perfringens 13124 Growth 24 hrs
Clostridium innocuum 14501 Growth 24 hrs
Clostridium sordellii 9714 Variable 24 hrs
Clostridium difficile 9689 Growth 24 hrs
Clostridium difficile 700057 Growth 24 hrs

Product Storage & Shelf Life

Storage: Upon receipt, store at room temperature in original package until used. Avoid overheating or freezing. Do not use media if there are signs of deterioration (shrinking, cracking, or discoloration due to oxidation of media) or contamination. The expiration date applies to the product in its original packaging and stored as directed. Do not use product past the expiration date shown on the label.

Shelf Life: 90 days from date of manufacture.