Brain Heart Infusion Agar with Horse Blood and Taurocholate (BHIY-HT) is an enriched non-selective media for the germination and cultivation of Clostridium difficile cells and spores along with other fastidious anaerobic microorganisms.
Brain Heart Infusion Agar w/ Horse Blood & Taurocholate – BHIY-HT
Product Description & Summary
BHIY-HT agar is an enriched non-selective media used for the isolation and cultivation of a wide variety of microorganisms, including yeast and molds. The basic nutritive properties are brain heart infusion, meat peptone, and yeast extract. Horse blood is added to stimulate growth. Sodium taurocholate is added to promote the germination of Clostridium difficile spores. When grown on this media, Clostridium difficile will exhibit characteristic colonial morphology, fluorescence, and smell. This media is prepared, dispensed, and packaged under oxygen-free conditions to prevent the formation of oxidized products prior to use.
Interpretation of Results & Limitations
This media supports good growth of many fastidious and non-fastidious anaerobes isolated from clinical specimens.
BHIY-HT will not provide complete information for identification of bacterial isolates. Additional test procedures and media are required for complete identification. Consult reference materials for additional information.
The following organisms are routinely used for quality control testing at Anaerobe Systems.
|Organism Tested||ATCC #||Results||Time|
|Bacteroides fragilis||25285||Growth||24 hrs|
|Enterococcus faecalis||29212||Growth||24 hrs|
|Clostridium sporogenes||3584||Growth||24 hrs|
|Clostridium beijerinckii||8260||Inhibited||24 hrs|
|Proteus mirabilis||12453||Growth||24 hrs|
|Clostridium perfringens||13124||Growth||24 hrs|
|Clostridium innocuum||14501||Growth||24 hrs|
|Clostridium sordellii||9714||Variable||24 hrs|
|Clostridium difficile||9689||Growth||24 hrs|
|Clostridium difficile||700057||Growth||24 hrs|
Product Storage & Shelf Life
Storage: Upon receipt, store at room temperature in original package until used. Avoid overheating or freezing. Do not use media if there are signs of deterioration (shrinking, cracking, or discoloration due to oxidation of media) or contamination. The expiration date applies to the product in its original packaging and stored as directed. Do not use product past the expiration date shown on the label.
Shelf Life: 90 days from date of manufacture.