Brain Heart Infusion Agar

Brain Heart Infusion Agar (BHI Agar)

Brain Heart Infusion (BHI) Agar is a general purpose enriched non-selective media for the cultivation of a wide variety of microorganisms, including yeasts, molds, and bacteria.

BHI Agar Plate

P. anaerobius (ATCC 27337) Growing On BHI Agar Plate

4 plates per package

Catalog #
AS-6426 BHI Mono Plate
4 plates
BHI Product InsertBHI SDS

Product Description & Summary

BHI Agar is an enriched non-selective media used for the isolation and cultivation of a wide variety of bacteria, including yeast and molds. The basic nutritive properties of this media are brain heart infusion from solids, meat peptone, and yeast extract. Dextrose provides a carbohydrate source for the fermentative microorganisms. Enrichment of the media with hemin and vitamin K1 is for the enhanced recovery of anaerobes. This media is prepared, dispensed, and packaged under oxygen-free conditions to prevent the formation of oxidized products prior to use.

Interpretation of Results & Limitations

This media supports good growth of many fastidious and non-fastidious anaerobes isolated from clinical specimens.

BHI Agar will not provide complete information for identification of bacterial isolates. Additional test procedures and media are required for complete identification. It is recommended that a non-selective media, such as Brucella Blood Agar (BRU, catalog #: AS-111) also be inoculated from the same clinical specimen to assure recovery of all species present. Consult reference materials for additional information.

Quality Control

The following organisms are routinely used for quality control testing at Anaerobe Systems.


Organism Tested ATCC # Results Time
Bacteroides fragilis 25285 Growth 24 hrs
Prevotella melaninogenica 25845 Growth 24 – 48 hrs
Fusobacterium mortiferum 25557 Growth 24 – 48 hrs
Fusobacterium necrophorum 25286 Growth 24 – 48 hrs
Clostridium perfringens 13124 Growth 24 hrs
Clostridium sporogenes 3584 Growth 24 hrs
Peptostreptococcus anaerobius 27337 Growth 24 hrs
Proteus mirabilis 12453 Growth 24 hrs
Propionibacterium acnes or

Clostridium difficile



Growth 24 – 48 hrs

24 hrs

Staphylococcus aureus or

Enterococcus faecalis



Growth 24 hrs

24 hrs

Escherichia coli 25922 Growth 24 hrs

Product Storage & Shelf Life

Storage:Upon receipt, store at room temperature in original package until used. Avoid overheating or freezing. Do not use media if there are signs of deterioration (shrinking, cracking, or discoloration due to oxidation of media) or contamination. The expiration date applies to the product in its original packaging and stored as directed. Do not use product past the expiration date shown on the label.

Shelf Life: 90 days from date of manufacture.