Chocolate Agar – CHOC

Chocolate Agar – CHOC

Chocolate Agar (CHOC) is recommended for use in the isolation and cultivation of fastidious aerobic and anaerobic organisms.

CHOC Agar Plate

N. gonorrheae (ATCC 43069) Growing On CHOC Agar Plate

1 or 4 plates per package

Catalog #
AS-214 Chocolate Agar (CHOC) 1 plate
AS-244 Chocolate Agar (CHOC) 4 plates

Non-stock item:
AS-214 – 100 pack minimum order, allow 2-4 weeks for delivery.
AS-244 – 25 pack minimum order, allow 2-4 weeks for delivery.

CHOC Product InsertCHOC SDS

Product Description & Summary

CHOC agar is an enriched non-selective media supplemented with cofactor V (nicotinamide adenine dinucleotide; NAD) and cofactor X (hemin), and facilitates the growth of fastidious anaerobes. CHOC agar is recommended for aerotolerance testing of obligate anaerobes. Heated horse blood added to the media gives it a “chocolate” appearance. This media is prepared, dispensed, and packaged under oxygen-free conditions to prevent the formation of oxidized products prior to use.

Interpretation of Results & Limitations

If used properly, this media should support good growth of most pathogens found in clinical infections including Haemophilus influenzae, Neisseria gonorrhoeae, and other fastidious aerobes and anaerobes in the correct environment. Microorganisms that grow on CHOC and incubated in 5% – 10% CO2 are not obligate anaerobes and findings should correlate with the microorganisms identified in the aerobic culture and direct Gram stain.

CHOC agar will not provide complete information for identification of bacterial isolates. Additional test procedures and media are required for complete identification. It is important to note that some anaerobic microorganisms can be aerotolerant, such as certain species of Clostridium, Actinomyces, Propionibacterium, Bifidobacterium, and Lactobacillus. These aerotolerant microorganisms typically grow well in anaerobic conditions but poorly in carbon dioxide or air. Consult reference materials for additional information.

Quality Control

The following organisms are routinely used for quality assurance performance testing at Anaerobe Systems.

Organism Tested ATCC # Results in 5% CO2 Results in anaerobic conditions Time
Fusobacterium necrophorum 25286 No Growth Growth 24 – 48 hrs.
Neisseria gonorrhoeae* 43069 Growth Inhibited Growth 48 hrs.
Haemophilus influenzae* 10211 Growth Growth 24 – 48 hrs.
Streptococcus pneumoniae 49619 Growth Growth 24 – 48 hrs.
Staphylococcus aureus 25923 Growth Growth 24 – 48 hrs.
Escherichia coli 25922 Growth Growth 24 hrs.

* Organisms specified by CLSI: M22-A3 for quality control performance testing of Chocolate Agar.

Product Storage & Shelf Life

Storage: Upon receipt, store at 2 – 8oC in original package until use. Avoid overheating or freezing. Do not use media if there are signs of deterioration (shrinking, cracking, or discoloration due to oxidation of media) or contamination. The expiration date applies to the product in its original packaging and stored as directed. Do not use product past the expiration date shown on the container.

Shelf Life: 6 months from date of manufacture.