Crystal Violet Erythromycin Agar (CVE) is an enriched, selective, and differential medium used for the isolation and presumptive identification of Fusobacterium nucleatum.
Crystal Violet-Erythromycin Agar – CVE
Product Description & Summary
CVE has been formulated with lower concentrations of pancreatic digest of casein and glucose than most media, to provide less optimal conditions for fastidious microorganisms. Tryptophan, yeast extract, and defibrinated sheep blood was added to increase the recovery of F. nucleatum from clinical specimens. This medium contains erythromycin and crystal violet at concentrations that inhibit most other anaerobes, but has no suppressive effects on F. nucleatum. When grown on CVE, F. nucleatum colonies grow to approximately 2.0 mm and has a blue pigmentation. This medium is prepared, dispensed, and packaged under oxygen-free conditions to prevent the formation of oxidized products prior to use.
Interpretation of Results & Limitations
If used properly, CVE will support good growth of Fusobacterium nucleatum found in clinical infections.
CVE will not provide complete information for identification of bacterial isolates. Additional test procedures and media are required for complete identification. It is recommended that a non-selective media, such as Brucella Blood Agar (BRU, catalog #: AS-111) also be inoculated from the same clinical specimen to assure recovery of all species present. Consult reference materials for additional information.
The following organisms are routinely used for quality assurance performance testing at Anaerobe Systems.
|Organism Tested||ATCC #||Results||Time||Special Reaction|
|Fusobacterium nucleatum||25586||Growth||24 hrs||Blue pigment|
|Aggregatibacter actinomycetemcomitans||29522||Growth||24 – 48 hrs|
|Porphyromonas asaccharolytica||25260||No Growth|
|Peptostreptococcus anaerobius||27337||No Growth|
|Actinomyces viscosus||43146||No Growth|
|Fusobacterium necrophorum||25286||Growth||24 hrs|
|Propionibacterium acnes||6919||No Growth|
|Clostridium difficile||9689||No Growth|
Product Storage & Shelf Life
Storage: Upon receipt, store at room temperature in original package until used. Avoid overheating or freezing. Do not use media if there are signs of deterioration (shrinking, cracking, or discoloration due to oxidation of media) or contamination. The expiration date applies to the product in its original packaging and stored as directed. Do not use product past the expiration date shown on the label.
Shelf Life: 90 days from date of manufacture.