Enriched Tryptic Soy Agar – ETSA

Enriched Tryptic Soy Agar – ETSA

Enriched Tryptic Soy Agar (ETSA) is an enriched, non-selective, and differential medium intended for the isolation, quantitation, and partial identification of many fastidious and non-fastidious microorganisms.

ETSA Agar Plate

P. melaninogenica (ATCC 25845) Growing On ETSA Agar Plate

4 plates per package

Catalog #
AS-548 ETSA mono plate 4 plates

Non-stock item: 25 pack minimum order, allow 2-4 weeks for delivery.

ETSA Product InsertETSA SDS

Product Description & Summary

ETSA agar is an enriched, non-selective, and differential medium. The nutritive base consists of casein, soy peptone, yeast extract, and dextrose. The media is supplemented with laked sheep blood and sheep serum to facilitate the recovery, and promote the black pigmentation of, Prevotella and Porphyromonas spp. Sodium formate and sodium fumarate were included as energy sources for microorganisms that require them for growth. Hemin and vitamin K1 is added for the enhanced recovery of anaerobic bacteria. This medium is prepared, dispensed, and packaged under oxygen-free conditions to prevent the formation of oxidized products prior to use.

Interpretation of Results & Limitations

This media supports good growth of many fastidious and non-fastidious anaerobes isolated from clinical specimens. It also facilitates the pigmentation of the black pigmenting Prevotella and Porphyromonas spp.

ETSA will not provide complete information for identification of bacterial isolates. Additional test procedures and media are required for complete identification. Consult reference materials for additional information.

Quality Control

The following organisms are routinely used for quality control testing at Anaerobe Systems.

Organism Tested ATCC # Results Time Special Reaction
Bacteroides fragilis 25285 Growth 24 – 48 hrs
Prevotella melaninogenica 25845 Growth 24 – 48 hrs Pigment*
Fusobacterium necrophorum 25286 Growth 24 hrs
Fusobacterium nucleatum 25586 Growth 24 – 48 hrs
Clostridium perfringens 13124 Growth 24 hrs
Peptostreptococcus anaerobius 27337 Growth 24 hrs
Prevotella intermedia 25611 Growth 24 hrs Pigment*
Propionibacterium acnes 6919 Growth 24 – 48 hrs
Eggerthella lenta 43055 Growth 24 hrs
Actinomyces viscosus 43146 Growth 24 – 48 hrs
Porphyromonas gingivalis 33277 Growth 24 – 72 hrs Pigment*

*Pigment production may require more than 48 hours of incubation.

Product Storage & Shelf Life

Storage: Upon receipt, store at room temperature in original package until used. Avoid overheating or freezing. Do not use media if there are signs of deterioration (shrinking, cracking, or discoloration due to oxidation of media) or contamination. The expiration date applies to the product in its original packaging and stored as directed. Do not use product past the expiration date shown on the label.

Shelf Life: 90 days from date of manufacture.