Enriched Tryptic Soy Agar with Nalidixic Acid and Vancomycin (ETSA-NV) is an enriched, selective, and differential medium intended for the cultivation and isolation of most gram-negative anaerobic and other fastidious bacteria.
Enriched Tryptic Soy Agar with Naladixic acid and Vancomycin – ETSA-NV
Product Description & Summary
ETSA-NV is an enriched, selective, and differential medium. The nutritive base consists of casein, soy peptone, yeast extract, and dextrose. The media is supplemented with laked sheep blood and sheep serum to facilitate the recovery, and promote the black pigmentation of, Prevotella and Porphyromonas spp. Sodium formate and sodium fumarate were included as energy sources for microorganisms that require them for growth. Hemin and vitamin K1 is added for the enhanced recovery of anaerobic bacteria. The selective agents, nalidixic acid and vancomycin, were added to inhibit most gram-positive anaerobes. This medium is prepared, dispensed, and packaged under oxygen-free conditions to prevent the formation of oxidized products prior to use.
Interpretation of Results & Limitations
This media supports good growth of many gram-negative anaerobes, along with other fastidious bacteria, isolated from clinical specimens. It also facilitates the pigmentation of the black pigmenting Prevotella and Porphyromonas spp.
ETSA-NV will not provide complete information for identification of bacterial isolates. Additional test procedures and media are required for complete identification. It is recommended that a non-selective media, such as Brucella Blood Agar (BRU, catalog #: AS-111) also be inoculated from the same clinical specimen to assure recovery of all species present. Consult reference materials for additional information.
The following organisms are routinely used for quality control testing at Anaerobe Systems.
|Organism Tested||ATCC #||Results||Time||Special Reaction|
|Bacteroides fragilis||25285||Growth||24 – 48 hrs|
|Prevotella melaninogenica||25845||Growth||24 – 48 hrs||Pigment*|
|Fusobacterium necrophorum||25286||Growth||24 hrs|
|Fusobacterium nucleatum||25586||Growth||24 – 48 hrs|
|Clostridium perfringens||13124||No Growth|
|Peptostreptococcus anaerobius||27337||No Growth|
|Prevotella intermedia||25611||Growth||24 hrs||Pigment*|
|Propionibacterium acnes||6919||No Growth|
|Eggerthella lenta||43055||No Growth|
|Actinomyces viscosus||43146||No Growth|
|Porphyromonas gingivalis||33277||Inhibit to No Growth|
*Pigment production may require more than 48 hours of incubation.
Product Storage & Shelf Life
Storage: Upon receipt, store at room temperature in original package until used. Avoid overheating or freezing. Do not use media if there are signs of deterioration (shrinking, cracking, or discoloration due to oxidation of media) or contamination. The expiration date applies to the product in its original packaging and stored as directed. Do not use product past the expiration date shown on the label.
Shelf Life: 90 days from date of manufacture.