Mitis-Salivarius Agar with Tellurite – MSAT

Mitis-Salivarius Agar with Tellurite – MSAT

Mitis Salivarius Agar with Tellurite (MSAT) is an enriched selective medium for the isolation of Streptococcus mitis, Streptococcus salivarius, and other oral Streptococci and Enterococci found in clinical specimens.

MSAT Agar Plate

S. salivarius (ATCC 13419) Growing On MSAT Agar Plate

4 plates per package

Catalog #
Description
Qty
Price
AS-544 MSAT mono plate 4 plates
$9.34
MSAT Product InsertMSAT SDS

Product Description & Summary

MSAT agar is an enriched selective medium used for isolating Streptococcus mitis, Streptococcus salivarius, and Enterococci. Peptones are added as source of carbon, nitrogen, vitamins, and minerals. Dextrose and saccharose are included as carbohydrate sources. The selective agents include crystal violet and potassium tellurite, which inhibit most gram-negative bacilli and most gram-positive bacteria except the Streptococci. Trypan blue gives the colonies a blue coloration. This media is prepared, dispensed, and packaged under oxygen-free conditions to prevent the formation of oxidized products prior to use.

Interpretation of Results & Limitations

If used properly, MSAT agar will support the growth of Streptococcus mitis, Streptococcus salivarius, and Enterococci from clinical specimens.

Quality Control

The following organisms are routinely used for quality control testing at Anaerobe Systems.

Organism Tested ATCC # Results Time Special Reaction
Bacteroides fragilis 25285 Inhibited to No Growth
Prevotella melaninogenica 25845 Inhibited to No Growth
Fusobacterium necrophorum 25286 Inhibited to No Growth
Fusobacterium nucleatum 25586 Inhibited to No Growth
Clostridium perfringens 13124 Variable
Enterococcus faecalis 29212 Growth 24 – 48 hrs Blue/Black colonies
Staphylococcus aureus 25923 Inhibited to No Growth
Streptococcus mitis 9811 Growth 24 hrs Blue colonies
Streptococcus pyogenes 19615 Growth 24 hrs Blue colonies
Streptococcus salivarius 13419 Growth 24 hrs Blue “gum drop” colonies
Streptococcus mutans 25175 Growth 24 – 48 hrs Blue colonies
Peptostreptococcus anaerobius 27337 Variable

Product Storage & Shelf Life

Storage: Upon receipt, store at room temperature in original package until used. Avoid overheating or freezing. Do not use media if there are signs of deterioration (shrinking, cracking, or discoloration due to oxidation of media) or contamination. The expiration date applies to the product in its original packaging and stored as directed. Do not use product past the expiration date shown on the label.

Shelf Life: 90 days from date of manufacture.