Mitis Salivarius Agar with Tellurite (MSAT) is an enriched selective and differential medium for the isolation of Streptococcus mitis, Streptococcus salivarius, and other oral Streptococci and Enterococci found in clinical specimens.
Mitis-Salivarius Agar with Tellurite – MSAT
Product Description & Summary
MSAT agar is an enriched selective medium used for isolating Streptococcus mitis, Streptococcus salivarius, and Enterococci. Peptones are added as source of carbon, nitrogen, vitamins, and minerals. Dextrose and saccharose are included as carbohydrate sources. The selective agents include crystal violet and potassium tellurite, which inhibit most gram-negative bacilli and most gram-positive bacteria except the Streptococci. Trypan blue gives the colonies a blue coloration. This media is prepared, dispensed, and packaged under oxygen-free conditions to prevent the formation of oxidized products prior to use.
Interpretation of Results & Limitations
If used properly, MSAT agar will support the growth of Streptococcus mitis, Streptococcus salivarius, and Enterococci from clinical specimens.
The following organisms are routinely used for quality control testing at Anaerobe Systems.
|Organism Tested||ATCC #||Results||Time||Special Reaction|
|Bacteroides fragilis||25285||Inhibited to No Growth|
|Prevotella melaninogenica||25845||Inhibited to No Growth|
|Fusobacterium necrophorum||25286||Inhibited to No Growth|
|Fusobacterium nucleatum||25586||Inhibited to No Growth|
|Enterococcus faecalis||29212||Growth||24 – 48 hrs||Blue/Black colonies|
|Staphylococcus aureus||25923||Inhibited to No Growth|
|Streptococcus mitis||9811||Growth||24 hrs||Blue colonies|
|Streptococcus pyogenes||19615||Growth||24 hrs||Blue colonies|
|Streptococcus salivarius||13419||Growth||24 hrs||Blue “gum drop” colonies|
|Streptococcus mutans||25175||Growth||24 – 48 hrs||Blue colonies|
Product Storage & Shelf Life
Storage: Upon receipt, store at room temperature in original package until used. Avoid overheating or freezing. Do not use media if there are signs of deterioration (shrinking, cracking, or discoloration due to oxidation of media) or contamination. The expiration date applies to the product in its original packaging and stored as directed. Do not use product past the expiration date shown on the label.
Shelf Life: 90 days from date of manufacture.