Anaerobic Enrichment Agar (MTGE) is an enriched non-selective medium used for the cultivation of most anaerobic bacteria and other fastidious microorganisms.
MTGE-Anaerobic Enrichment Agar – MTGE
Product Description & Summary
MTGE is an enriched non-selective medium developed at Anaerobe Systems for the cultivation and isolation of most anaerobic bacteria and other fastidious microorganisms. A protein formulation and yeast extract comprise the basal medium, which is then supplemented with vitamin K1, calf serum, and volatile fatty acids as growth factors for many anaerobic bacteria. This media is prepared, dispensed, and packaged under oxygen-free conditions to prevent the formation of oxidized products prior to use.
Interpretation of Results & Limitations
If used properly, MTGE will support he good growth of many fastidious and non-fastidious anaerobes isolated from clinical specimens.
MTGE agar will not provide complete information for identification of bacterial isolates. Additional test procedures and media are required for complete identification. In some cases, MTGE agar may be overgrown with swarming Proteus spp. or Clostridium spp. It is recommended that selective media such as Laked Brucella Blood Agar with Kanamycin and Vancomycin (LKV, catalog #: AS-112) and/or Brucella Blood Agar with Phenylethyl Alcohol (PEA, catalog #: AS-113) also be inoculated from clinical specimens to prevent such overgrowth and thus provide isolated colonies. Consult reference materials for additional information.
The following organisms are routinely used for quality control testing at Anaerobe Systems.
|Organism Tested||ATCC #||Results||Time|
|Campylobacter ureolyticus||33387||Growth||24 hrs|
|Bacteroides fragilis||25285||Growth||24 hrs|
|Bacteroides vulgatus||8482||Growth||24 hrs|
|Prevotella melaninogenica||25845||Growth||24 hrs|
|Fusobacterium necrophorum||25286||Growth||24 hrs|
|Fusobacterium nucleatum||25586||Growth||24 hrs|
|Clostridium perfringens||13124||Growth||24 hrs|
|Peptostreptococcus anaerobius||27337||Growth||24 hrs|
|Clostridium novyi Type A||7659||Growth||24 hrs|
|Propionibacterium acnes||6919||Growth||24 hrs|
|Staphylococcus aureus||25923||Growth||24 hrs|
|Porphyromonas gingivalis||33277||Growth||48 – 72 hrs|
|Prevotella intermedia||25611||Growth||24 – 48 hrs|
Product Storage & Shelf Life
Storage: Upon receipt, store at room temperature in original package until used. Avoid overheating or freezing. Do not use media if there are signs of deterioration (shrinking, cracking, or discoloration due to oxidation of media) or contamination. The expiration date applies to the product in its original packaging and stored as directed. Do not use product past the expiration date shown on the label.
Shelf Life: 90 days from date of manufacture.