MTGE-Anaerobic Enrichment Agar – MTGE

MTGE-Anaerobic Enrichment Agar – MTGE

Anaerobic Enrichment Agar (MTGE) is an enriched non-selective medium used for the cultivation of most anaerobic bacteria and other fastidious microorganisms.

MTGE Agar Plate

B. fragilis (ATCC 25285) Growing On MTGE Agar Plate

4 plates per package

Catalog #
AS-777 MTGE mono plate 4 plates

Non-stock item: 25 pack minimum order, allow 2-4 weeks for delivery.

MTGE Product InsertMTGE SDS

Product Description & Summary

MTGE is an enriched non-selective medium developed at Anaerobe Systems for the cultivation and isolation of most anaerobic bacteria and other fastidious microorganisms. A protein formulation and yeast extract comprise the basal medium, which is then supplemented with vitamin K1, calf serum, and volatile fatty acids as growth factors for many anaerobic bacteria. This media is prepared, dispensed, and packaged under oxygen-free conditions to prevent the formation of oxidized products prior to use.

Interpretation of Results & Limitations

If used properly, MTGE will support he good growth of many fastidious and non-fastidious anaerobes isolated from clinical specimens.

MTGE agar will not provide complete information for identification of bacterial isolates. Additional test procedures and media are required for complete identification. In some cases, MTGE agar may be overgrown with swarming Proteus spp. or Clostridium spp. It is recommended that selective media such as Laked Brucella Blood Agar with Kanamycin and Vancomycin (LKV, catalog #: AS-112) and/or Brucella Blood Agar with Phenylethyl Alcohol (PEA, catalog #: AS-113) also be inoculated from clinical specimens to prevent such overgrowth and thus provide isolated colonies. Consult reference materials for additional information.

Quality Control

The following organisms are routinely used for quality control testing at Anaerobe Systems.

Organism Tested ATCC # Results Time
Campylobacter ureolyticus 33387 Growth 24 hrs
Bacteroides fragilis 25285 Growth 24 hrs
Bacteroides vulgatus 8482 Growth 24 hrs
Prevotella melaninogenica 25845 Growth 24 hrs
Fusobacterium necrophorum 25286 Growth 24 hrs
Fusobacterium nucleatum 25586 Growth 24 hrs
Clostridium perfringens 13124 Growth 24 hrs
Peptostreptococcus anaerobius 27337 Growth 24 hrs
Clostridium novyi Type A 7659 Growth 24 hrs
Propionibacterium acnes 6919 Growth 24 hrs
Staphylococcus aureus 25923 Growth 24 hrs
Porphyromonas gingivalis 33277 Growth 48 – 72 hrs
Prevotella intermedia 25611 Growth 24 – 48 hrs

Product Storage & Shelf Life

Storage: Upon receipt, store at room temperature in original package until used. Avoid overheating or freezing. Do not use media if there are signs of deterioration (shrinking, cracking, or discoloration due to oxidation of media) or contamination. The expiration date applies to the product in its original packaging and stored as directed. Do not use product past the expiration date shown on the label.

Shelf Life: 90 days from date of manufacture.