Oral H2S Organisms Agar with Lead Acetate (OHO-C) is intended for the identification of volatile sulfur compound, primarily hydrogen sulfide, producing bacteria found in clinical specimens.
Oral Hydrogen Sulfide Organism Carbohydrate Agar – OHO-C
Product Description & Summary
OHO-C agar is an enriched differential medium used for the detection of hydrogen sulfide producing bacteria from clinical specimens. The nutritive base, Columbia agar, is supplemented with glutathione and lead acetate. Glutathione is added as a substrate for hydrogen sulfide production by the organism. Lead acetate reacts with hydrogen sulfide to produce a black lead sulfide precipitate, that develops within the colonies or on the agar surrounding them. This medium is prepared, stored, and dispensed under oxygen-free conditions to prevent the formation of oxidized products prior to use.
Interpretation of Results & Limitations
If used properly, OHO-C will support good growth and hydrogen sulfide detection of oral anaerobic bacteria from clinical specimens. Black to grey halos surrounding the colonies, or black color in the center of colonies, usually signifies hydrogen sulfide production.
OHO-C will not provide complete information for identification of bacterial isolates. Additional test procedures and media are required for complete identification. It is recommended that a non-selective media, such as Brucella Blood Agar (BRU, catalog #: AS-111) also be inoculated from the same clinical specimen to assure recovery of all species present. Consult reference materials for additional information.
The following organisms are routinely used for quality assurance performance testing at Anaerobe Systems.
|Organism Tested||ATCC #||Results||Time||Special Reaction|
|Bacteroides fragilis||25285||Growth||24 hrs|
|Prevotella melaninogenica||25845||Growth||24 – 48 hrs|
|Fusobacterium necrophorum||25286||Growth||24 hrs||+ H2S*|
|Fusobacterium nucleatum||25586||Growth||24 – 48 hrs||+ H2S*|
|Clostridium perfringens||13124||Growth||24 hrs||+ H2S*|
|Peptostreptococcus anaerobius||27337||Growth||24 – 48 hrs|
|Peptostreptococcus magnus||29328||Growth||24 hrs|
|Propionibacterium acnes or Clostridium difficile||6919
|24 – 48 hrs
|Enterococcus faecalis||29212||Growth||24 hrs|
|Campylobacter rectus||33238||Growth||24 – 48 hrs|
|Veillonella parvula||10790||Growth||24 hrs||+ H2S*|
*H2S production may require extended periods of incubation.
Product Storage & Shelf Life
Storage: Upon receipt, store at room temperature in original package until used. Avoid overheating or freezing. Do not use media if there are signs of deterioration (shrinking, cracking, or discoloration due to oxidation of media) or contamination. The expiration date applies to the product in its original packaging and stored as directed. Do not use product past the expiration date shown on the label.
Shelf Life: 90 days from date of manufacture.