Reinforced Clostridial Agar (RCA) is an enriched non-selective medium intended for the cultivation of Clostridia and other anaerobic and facultative bacteria from various clinical and non-clinical specimens.
REINFORCED CLOSTRIDIAL AGAR – RCA
Product Description & Summary
RCA agar is an enriched non-selective medium formulated by Hirsch and Grinstead to enhance the growth of clostridia from a small inoculum. Casein, beef extract, and yeast extract form the nutritive base that supplies a source of carbon, nitrogen, vitamins, and minerals. Dextrose is added as a source of carbohydrates. L-Cysteine is included as a reducing agent. Soluble starch is a detoxifier of metabolic by-products while sodium acetate acts as a buffer. This media is prepared, dispensed, and packaged under oxygen-free conditions to prevent the formation of oxidized products prior to use.
Interpretation of Results & Limitations
If used properly, RCA agar will support the good growth of Clostridia and many other anaerobes isolated from clinical or non-clinical specimens.
RCA agar will not provide complete information for identification of bacterial isolates. Additional test procedures and media are required for complete identification. It is recommended that a non-selective media, such as Brucella Blood Agar (BRU, catalog #: AS-111) also be inoculated from the same clinical specimen to assure recovery of all species present. Consult reference materials for additional information.
The following organisms are routinely used for quality control testing at Anaerobe Systems.
|Organism Tested||ATCC #
|Bacteroides fragilis||25285||Growth||24 hrs|
|Propionibacterium acnes||6919||Growth||24 – 48 hrs|
|Enterococcus faecalis||29212||Growth||24 hrs|
|Clostridium difficile||9689||Growth||24 hrs|
|Clostridium difficile||700057||Growth||24 hrs|
|Clostridium perfringens||13124||Growth||24 hrs|
|Clostridium innocuum||14501||Growth||24 hrs|
|Clostridium sporogenes||3584||Growth||24 hrs|
|Clostridium beijerinckii||8260||Growth||24 hrs|
|Clostridium sordellii||9714||Growth||24 hrs|
Product Storage & Shelf Life
Storage: Upon receipt, store at room temperature in original package until used. Avoid overheating or freezing. Do not use media if there are signs of deterioration (shrinking, cracking, or discoloration due to oxidation of media) or contamination. The expiration date applies to the product in its original packaging and stored as directed. Do not use product past the expiration date shown on the label.
Shelf Life: 90 days from date of manufacture.