Tryptic Soy Agar with N-acetylmuramic acid (TSA-NAM) is an enriched medium used for the isolation and presumptive identification of periodontal pathogens, including Tannerella forsythia.
Tryptic Soy Agar with n-Acetylmuramic acid – TSA-NAM
Product Description & Summary
TSA-NAM is an enriched medium for the isolation and cultivation of Tannerella forsythia. The nutritive base consists of casein and soy peptone. Hemin and vitamin K1 are added for the recovery of some fastidious anaerobes. This media is supplement with sheep blood and N-acetylmuramic acid, which are necessary growth factors for Tannerella forsythia. This media is prepared, dispensed, and packaged under oxygen-free conditions to prevent the formation of oxidized products prior to use.
Interpretation of Results & Limitations
If used properly, TSA-NAM will support good growth of Tannerella forsythia found in clinical specimens.
TSA-NAM will not provide complete information for identification of bacterial isolates. Additional test procedures and media are required for complete identification. It is recommended that a enriched media, such as Brucella Blood Agar (BRU, catalog #: AS-111) also be inoculated from the same clinical specimen to assure recovery of all species present. Consult reference materials for additional information.
The following organisms are routinely used for quality control testing at Anaerobe Systems.
|Organism Tested||ATCC #||Results||Time|
|Bacteroides fragilis||25285||Growth||24 hrs|
|Prevotella melaninogenica||25845||Growth||24 – 48 hrs|
|Fusobacterium nucleatum||25586||Growth||24 – 48 hrs|
|Clostridium perfringens||13124||Growth||24 hrs|
|Peptostreptococcus anaerobius||27337||Growth||24 hrs|
|Tannerella forsythia||43037||Growth||48 – 72 hrs|
|Escherichia coli||25922||Growth||24 hrs|
Product Storage & Shelf Life
Storage: Upon receipt, store at room temperature in original package until used. Avoid overheating or freezing. Do not use media if there are signs of deterioration (shrinking, cracking, or discoloration due to oxidation of media) or contamination. The expiration date applies to the product in its original packaging and stored as directed. Do not use product past the expiration date shown on the label.
Shelf Life: 90 days from date of manufacture.