Tryptic Soy Agar with Tween®andLecithin (TSA-TL) is an enriched nonselective medium used for the isolation, cultivation, and enumeration of microorganisms, including yeast and molds.
Tryptic Soy Agar with Tween and Lecithin mono plate 25ml fill (TSA-TL)
Product Description & Summary
TSA-TL is an enriched nonselective medium used for the detection, isolation, and enumeration of many microorganisms, including yeast and molds. The nutritive base consists of casein and soy peptone. Sodium chloride maintains the osmotic equilibrium of the media. Tween 80® and lecithin, two commonly used neutralizers, are reported to inactivate residual disinfectants when the sample is being collected. Lecithin neutralizes quaternary ammonium compounds, while Tween 80® neutralizes substituted phenolic disinfectants. TSA-TL may also be used in environmental air sampling protocols. This media is prepared, dispensed, and packaged under oxygen-free conditions to prevent the formation of oxidized products prior to use.
Interpretation of Results & Limitations
If used properly, TSA-TL will support good growth of many microorganism, including yeast and molds.
TSA-TL will not provide complete information for identification of microorganisms, including yeast and mold isolates. Additional test procedures and media are required for complete identification. This medium may not support the growth of all clinically significant anaerobes. Consult reference materials for additional information.
The following organisms are routinely used for quality control testing at Anaerobe Systems.
|Organism Tested||ATCC #||Results||Time|
|Saccharomyces cerevisiae||7754||Growth||24 – 48 hrs|
|Candidia albicans||11006||Growth||24 – 48 hrs|
|Proteus mirabilis||12453||Growth||24 – 48 hrs|
|Enterococcus faecalis||29212||Growth||24 – 48 hrs|
|Escherichia coli||25922||Growth||24 – 48 hrs|
|Staphylococcus aureus||25923||Growth||24 – 48 hrs|
|Staphylococcus epidermiditis||14990||Growth||24 – 48 hrs|
|Bacillus subtilis||6051||Growth||24 – 48 hrs|
|Pseudomonas aeruginosa||27853||Growth||24 – 48 hrs|
Product Storage & Shelf Life
Storage: Upon receipt, store at room temperature in original package until used. Avoid overheating or freezing. Do not use media if there are signs of deterioration (shrinking, cracking, or discoloration due to oxidation of media) or contamination. The expiration date applies to the product in its original packaging and stored as directed. Do not use product past the expiration date shown on the label.
Shelf Life: 6 months from date of manufacture.