Tryptic Soy Blood Agar (TSBA) is an enriched nonselective medium used for the isolation and cultivation of many fastidious and non-fastidious microorganisms found in clinical specimens. This medium is also suitable for use in the CAMP test for the presumptive identification of group B Streptococci.
Tryptic Soy Blood Agar – TSBA
Product Description & Summary
TSBA is an enriched nonselective medium used for the isolation and cultivation of many microorganisms. The nutritive base consists of casein and soy peptone, which has been supplemented with sheep blood for the observation of hemolytic reactions, like the typical double zone β-hemolytic reactions of Clostridium perfringens. The media is supplemented with hemin and vitamin K1 for the enhanced recovery of some fastidious anaerobes. This media is prepared, dispensed, and packaged under oxygen-free conditions to prevent the formation of oxidized products prior to use.
Interpretation of Results & Limitations
If used properly, TSBA will support good growth or many fastidious microorganisms found in clinical infections. In addition, this media will support the typical double zone of β-hemolysis around colonies of Clostridium perfringens.
TSBA agar will not provide complete information for identification of bacterial isolates. Additional test procedures and media are required for complete identification. In some cases, TSBA agar may be overgrown with swarming Proteus spp. or Clostridium spp. It is recommended that selective media such as Brucella Laked Blood Agar with Kanamycin and Vancomycin (LKV, catalog #: AS-112) and/or Brucella Blood Agar with Phenylethyl Alcohol (PEA, catalog #: AS-113) also be inoculated from clinical specimens to prevent such overgrowth and thus provide isolated colonies. Consult reference materials for additional information.
The following organisms are routinely used for quality control testing at Anaerobe Systems.
|Organism Tested||ATCC #||Results||Time||Special Reaction|
|Bacteroides fragilis*||25285||Growth||24 hrs|
|Prevotella melaninogenica*||25845||Growth||48 hrs|
|Fusobacterium nucleatum*||25586||Growth||24 – 48 hrs|
|Clostridium perfringens*||13124||Growth||24 hrs||Double Zone of β-hemolysis|
|Peptostreptococcus anaerobius*||27337||Growth||24 – 48 hrs|
|Staphylococcus aureus or Enterococcus faecalis||25923 29212||Growth||24 hrs|
|Escherichia coli||25922||Growth||24 hrs|
|Proteus mirabilis||12453||Growth||24 hrs|
|Propionibacterium acnes or Clostridium difficile||6919 9689||Growth||24 – 48 hrs
24 – 48 hrs
* Organisms specified by CLSI for Quality Control testing of Anaerobic Blood Agars.
Product Storage & Shelf Life
Storage: Upon receipt, store at room temperature in original package until used. Avoid overheating or freezing. Do not use media if there are signs of deterioration (shrinking, cracking, or discoloration due to oxidation of media) or contamination. The expiration date applies to the product in its original packaging and stored as directed. Do not use product past the expiration date shown on the label.
Shelf Life: 90 days from date of manufacture.