Tryptic Soy Serum Bacitracin Vancomycin Agar (TSBV) is an enriched selective medium used for the isolation and presumptive identification of Aggregatibacter actinomycetemcomitans, from clinical specimens.
Tryptic Soy-Serum-Bacitracin-Vancomycin Agar – TSBV
Product Description & Summary
TSBV agar is an enriched selective medium used for the isolation of Aggregatibacter actinomycetemcomitans from clinical specimens. The nutritive base consists of casein, soy peptone, and yeast extract. Sodium chloride is added to maintain osmotic equilibrium of the media. Serum is included to enhance the recovery of Aggregatibacter actinomycetemcomitans. The selective agents include bacitracin and vancomycin at concentrations that inhibit most gram-positive and gram-negative anaerobes. This media is prepared, dispensed, and packaged under oxygen-free conditions to prevent the formation of oxidized products prior to use.
Interpretation of Results & Limitations
If used properly, TSBV agar supports good growth of some of Aggregatibacter actinomycetemcomitans found in clinical infections.
TSBV will not provide complete information for identification of bacterial isolates. Additional test procedures and media are required for complete identification. It is recommended that a non-selective media, such as Brucella Blood Agar (BRU, catalog #: AS-111) also be inoculated from the same clinical specimen to assure recovery of all species present. Consult reference materials for additional information.
The following organisms are routinely used for quality control testing at Anaerobe Systems.
|Organism Tested||ATCC #||Results||Time|
|Aggregatibacter actinomycetemcomitans||29522||Growth||24 – 48 hrs|
|Actinomyces viscosus||43146||No Growth|
|Fusobacterium nucleatum||25586||Growth||24 – 48 hrs|
|Propionibacterium acnes||6919||No Growth|
|Clostridium difficile||9689||No Growth|
|Enterococcus faecalis||29212||No Growth|
Product Storage & Shelf Life
Storage: Upon receipt, store at room temperature in original package until used. Avoid overheating or freezing. Do not use media if there are signs of deterioration (shrinking, cracking, or discoloration due to oxidation of media) or contamination. The expiration date applies to the product in its original packaging and stored as directed. Do not use product past the expiration date shown on the label.
Shelf Life: 90 days from date of manufacture.