Tryptic Soy-Serum-Bacitracin-Vancomycin Agar – TSBV

Tryptic Soy-Serum-Bacitracin-Vancomycin Agar – TSBV

Tryptic Soy Serum Bacitracin Vancomycin Agar (TSBV) is an enriched selective medium used for the isolation and presumptive identification of Aggregatibacter actinomycetemcomitans, from clinical specimens.

TSBV Agar Plate

A. actinomycetemcomitans (ATCC 29522) Growing On TSBV Agar Plate

4 plates per package

Catalog #
AS-648 TSBV mono plate 4 plates

Non-stock item: 25 pack minimum order, allow 2-4 weeks for delivery.

TSBV Product InsertTSBV SDS

Product Description & Summary

TSBV agar is an enriched selective medium used for the isolation of Aggregatibacter actinomycetemcomitans from clinical specimens. The nutritive base consists of casein, soy peptone, and yeast extract. Sodium chloride is added to maintain osmotic equilibrium of the media. Serum is included to enhance the recovery of Aggregatibacter actinomycetemcomitans. The selective agents include bacitracin and vancomycin at concentrations that inhibit most gram-positive and gram-negative anaerobes. This media is prepared, dispensed, and packaged under oxygen-free conditions to prevent the formation of oxidized products prior to use.

Interpretation of Results & Limitations

If used properly, TSBV agar supports good growth of some of Aggregatibacter actinomycetemcomitans found in clinical infections.

TSBV will not provide complete information for identification of bacterial isolates. Additional test procedures and media are required for complete identification. It is recommended that a non-selective media, such as Brucella Blood Agar (BRU, catalog #: AS-111) also be inoculated from the same clinical specimen to assure recovery of all species present. Consult reference materials for additional information.

Quality Control

The following organisms are routinely used for quality control testing at Anaerobe Systems.

Organism Tested ATCC # Results Time
Aggregatibacter actinomycetemcomitans 29522 Growth 24 – 48 hrs
Actinomyces viscosus 43146 No Growth
Fusobacterium nucleatum 25586 Growth 24 – 48 hrs
Propionibacterium acnes 6919 No Growth
Clostridium difficile 9689 No Growth
Enterococcus faecalis 29212 No Growth


Product Storage & Shelf Life

Storage: Upon receipt, store at room temperature in original package until used. Avoid overheating or freezing. Do not use media if there are signs of deterioration (shrinking, cracking, or discoloration due to oxidation of media) or contamination. The expiration date applies to the product in its original packaging and stored as directed. Do not use product past the expiration date shown on the label.

Shelf Life: 90 days from date of manufacture.