Anaerobic Transport Medium (ATM and ATMSP) and Anaerobic Dental Transport Medium (DTM) are mineral salt based semi-solid medias with reducing agents. Designed as a holding medium to maintain the viability of microorganisms, both anaerobic and aerobic, through collection, transportation, and shipment of clinical specimens.
Anaerobic Transport Medium – ATM and ATMSP
Product Description & Summary
ATM, ATMSP, and DTM contain buffered mineral salts in a semi-solid media, with sodium thioglycollate and cysteine added to provide a reduced environment. This combination has been prepared to provide an environment which maintains the viability of most microorganisms without significant multiplication and allows for dilution of inhibitors present in clinical material. This medium is designed to meet the stringent viability requirements of obligate anaerobes. All tubes are supplied with hungate caps (screw caps containing a rubber septa), which allows for either direct injection of aspirated clinical specimens or introduction of tissue samples. Resazurin is added as an indicator of significant oxygen exposure to the media. This media is prepared, dispensed, and packaged under oxygen-free conditions.
Interpretation of Results & Limitations
Results for the recovery of bacteria will largely depend on proper and adequate specimen collection, timely transport, and processing in the laboratory. If used properly, this medium should maintain the viability of microorganisms, anaerobic and aerobic, present within a clinical specimen until transported and processed within the laboratory.
ATM, ATMSP, and DTM are designed as holding mediums to maintain viability of microorganisms contained within a specimen during transport. This medium will not provide complete information for identification of bacterial isolates. Additional test procedures and media are required for complete identification. Specimens should be transported and processed in the laboratory in a timely manner as delays may result in overgrowth by one organism present in a specimen from polymicrobic infections. Consult reference materials for additional information.
The following organisms are routinely used for quality assurance testing at Anaerobe Systems. To determine the holding capacity of ATM, ATMSP, and DTM, an ATCC isolate strain (listed below), from 24-hour growth, is inoculated into the media aerobically and held for 48 hours at room temperature. Each organism is streaked onto Anaerobic Brucella Blood Agar (BRU, catalog #: AS-111) in an anaerobic environment to obtain isolated colonies. Plates are incubated at 35–37oC for 48 hours and growth is observed.
|Organism Tested||ATCC #
|Bacteroides fragilis*||25285||Growth||24 hrs|
|Prevotella melaninogenica*||25845||Growth||24 – 48 hrs|
|Propionibacterium acnes*||6919||Growth||24 – 48 hrs|
|Fusobacterium nucleatum*||25586||Growth||24 hrs|
|Peptostreptococcus anaerobius*||27337||Growth||24 hrs|
|* Organisms specified by CLSI for Quality Control testing of Anaerobic Microbiological Transport Systems|
Product Storage & Shelf Life
Storage: Upon receipt, store at room temperature in original package until used. Avoid overheating or freezing. Do not use medium if there are signs of deterioration (shrinking, cracking or discoloration due to oxidation of media) or contamination. The expiration date applies to the product in its original packaging and stored as directed. Do not use product past the expiration date shown on the label.
Shelf Life: 1 year from date of manufacture